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dc.contributor.authorRodríguez Lombardero, Silvia
dc.contributor.authorRodríguez-Belmonte, Esther
dc.contributor.authorGonzález-Siso, María-Isabel
dc.contributor.authorVizoso-Vázquez, Ángel
dc.contributor.authorValdiglesias, Vanessa
dc.contributor.authorLaffon, Blanca
dc.contributor.authorCerdán, María Esperanza
dc.date2014
dc.date.accessioned2017-09-26T11:19:29Z
dc.date.available2017-09-26T11:19:29Z
dc.date.issued2014
dc.identifier.citationRodríguez-Lombardero, S.; Rodríguez-Belmonte, M.E.; González-Siso, M.I.; Vizoso-Vázquez, Á.; Valdiglesias, V.; Laffón, B.; Cerdán, M.E. Proteomic Analyses Reveal that Sky1 Modulates Apoptosis and Mitophagy in Saccharomyces cerevisiae Cells Exposed to Cisplatin. Int. J. Mol. Sci. 2014, 15, 12573-12590.es_ES
dc.identifier.issn1422-0067
dc.identifier.urihttp://hdl.handle.net/2183/19536
dc.description.abstract[Abstract] Sky1 is the only member of the SR (Serine–Arginine) protein kinase family in Saccharomyces cerevisiae. When yeast cells are treated with the anti-cancer drug cisplatin, Sky1 kinase activity is necessary to produce the cytotoxic effect. In this study, proteome changes in response to this drug and/or SKY1 deletion have been evaluated in order to understand the role of Sky1 in the response of yeast cells to cisplatin. Results reveal differential expression of proteins previously related to the oxidative stress response, DNA damage, apoptosis and mitophagy. With these precedents, the role of Sky1 in apoptosis, necrosis and mitophagy has been evaluated by flow-cytometry, fluorescence microscopy, biosensors and fluorescence techniques. After cisplatin treatment, an apoptotic-like process diminishes in the ∆sky1 strain in comparison to the wild-type. The treatment does not affect mitophagy in the wild-type strain, while an increase is observed in the ∆sky1 strain. The increased resistance to cisplatin observed in the ∆sky1 strain may be attributable to a decrease of apoptosis and an increase of mitophagy.es_ES
dc.description.sponsorshipMinisterio de Ciencia e Innovación; BFU2009-08854es_ES
dc.language.isoenges_ES
dc.publisherMultidisciplinary Digital Publishing Institutees_ES
dc.relation.urihttp://dx.doi.org/10.3390/ijms150712573es_ES
dc.rightsReconocimiento 3.0es_ES
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/
dc.subjectBmh1es_ES
dc.subjectBmh2es_ES
dc.subjectChemoresistancees_ES
dc.subjectYeastes_ES
dc.subjectCanceres_ES
dc.titleProteomic Analyses Reveal that Sky1 Modulates Apoptosis and Mitophagy in Saccharomyces cerevisiae Cells Exposed to Cisplatines_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.rights.accessinfo:eu-repo/semantics/openAccesses_ES
UDC.journalTitleInternational Journal of Molecular Scienceses_ES
UDC.volume15es_ES
UDC.issue7es_ES
UDC.startPage12573es_ES
UDC.endPage12590es_ES


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