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Bioprospecting for Thermozymes and Characterization of a Novel Lipolytic Thermozyme Belonging to the SGNH/GDSL Family of Hydrolases
(MDPI, 2022-05-20)
[Abstract] Functional screenings were conducted on two metagenomic libraries from hot springs in order to find novel thermozymes with potential biotechnological applications. These included enzymes acting on plant cell ...
Reactivity of a Recombinant Esterase from Thermus thermophilus HB27 in Aqueous and Organic Media
(MDPI, 2022-04-27)
[Abstract] The thermoalkalophilic membrane-associated esterase E34Tt from Thermus thermophilus HB27 was cloned and expressed in Kluyveromyces lactis (KLEST-3S esterase). The recombinant enzyme was tested as a biocatalyst ...
Cellulases from thermophiles found by metagenomics
(MDPI, 2018-07-10)
[Abstract] Cellulases are a heterogeneous group of enzymes that synergistically catalyze the hydrolysis of cellulose, the major component of plant biomass. Such reaction has biotechnological applications in a broad spectrum ...
Test for the Production and Assay of the Proteolytic Activities of Halophilic Bacteria and Archaea Isolated from Algerian Hypersaline Environments
(MDPI, 2020-12-24)
[Abstract] The present work was carried out on 133 halophilic strains isolated on MGM (Modified Growth Medium) medium with 12 and 23% (w/v) of salt. A screening of the extracellular proteolytic activities, carried out on ...
Exploring the Taxonomical and Functional Profile of As Burgas Hot Spring Focusing On Thermostable β-galactosidases
(Springer Nature, 2021-01-08)
[Abstract] In the present study we investigate the microbial community inhabiting As Burgas geothermal spring, located in Ourense (Galicia, Spain). The approximately 23 Gbp of Illumina sequences generated for each replicate ...
Biochemical and structural characterization of a novel thermophilic esterase EstD11 provide catalytic insights for the HSL family
(Elsevier B.V. on behalf of Research Network of Computational and Structural Biotechnology, 2021)
[Abstract]: A novel esterase, EstD11, has been discovered in a hot spring metagenomic library. It is a thermophilic and thermostable esterase with an optimum temperature of 60 C. A detailed substrate preference analysis ...
Ixr1 Regulates Ribosomal Gene Transcription and Yeast Response to Cisplatin
(Springer Nature, 2018-02-15)
[Abstract] Ixr1 is a Saccharomyces cerevisiae HMGB protein that regulates the hypoxic regulon and also controls the expression of other genes involved in the oxidative stress response or re-adaptation of catabolic and ...
The HMGB Protein KlIxr1, a DNA Binding Regulator of Kluyveromyces lactis Gene Expression Involved in Oxidative Metabolism, Growth, and dNTP Synthesis
(MDPI, 2021-08-21)
[Abstract] In the traditional fermentative model yeast Saccharomyces cerevisiae, ScIxr1 is an HMGB (High Mobility Group box B) protein that has been considered as an important regulator of gene transcription in response ...
Role of the E3 Ubiquitin-Ligase Hakai in Intestinal Inflammation and Cancer Bowel Disease
(Springer Nature, 2022-10-20)
[Abstract] The E3 ubiquitin-ligases are important for cellular protein homeostasis and their deregulation is implicated in cancer. The E3 ubiquitin-ligase Hakai is involved in tumour progression and metastasis, through the ...