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Copper toxicity on the marine microalga Phaeodactylum tricornutum: effects on photosynthesis and related parameters
dc.contributor.author | Cid, Ángeles | |
dc.contributor.author | Herrero, Concepción | |
dc.contributor.author | Torres, Enrique | |
dc.contributor.author | Abalde, Julio | |
dc.date.accessioned | 2014-10-21T18:27:16Z | |
dc.date.available | 2014-10-21T18:27:16Z | |
dc.date.issued | 1995-02 | |
dc.identifier.citation | Cid A, Herrero C, Torres E, Abalde J. Copper toxicity on the marine microalga phaeodactylum tricornutum: Effects on photosynthesis and related parameters. Aquatic Toxicology. 1995;31(2):165-74. | es_ES |
dc.identifier.uri | http://hdl.handle.net/2183/13715 | |
dc.description.abstract | Essential heavy metals, as copper, can be toxic for microalgae at high concentrations. Copper affected growth and other parameters closely related to photosynthesis of the marine diatom Phaeodactylum tricornutum. A copper concentration of 0.10 mg l−1 provoked about 50% growth reduction and 1 mg l−1inhibited the growth. Copper also interfered with photosynthesis and ATP production. A copper concentration of 0.5 mg l−1 reduced in a 50% the photosynthetic rate. Therefore, growth is more affected by copper than photosynthesis. Results of chlorophyll a fluorescence obtained by flow cytometry showed that copper's inhibitory effect on PS II activity is located on its oxidizing side. The lower copper concentration assayed provoked a significant decrease in the cellular pool of ATP. Pigment analysis by HPLC showed that copper affected the pigment pattern of P. tricornutum. Important changes were observed for chlorophyll a and its allomer: chlorophyll a proportion decreased while its allomer increased with the copper concentration, being maximum at 1 mg Cu l−1. The study of the intracellular pH by flow cytometry revealed that P. tricornutumcells exposed to 0.5 and 1 mg Cu l−1 showed an intracellular pH higher than control cultures cells, explaining the high proportion of the chlorophyll a allomer in these cells. | es_ES |
dc.description.sponsorship | Ministerio de Educación y Ciencia; PB89-0546 | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Elsevier | es_ES |
dc.relation.uri | http://dx.doi.org/10.1016/0166-445x(94)00071-w | es_ES |
dc.subject | Copper toxicity | es_ES |
dc.subject | Phaeodactylum tricornutum | es_ES |
dc.subject | Photosynthesis | es_ES |
dc.subject | Flow cytometry | es_ES |
dc.title | Copper toxicity on the marine microalga Phaeodactylum tricornutum: effects on photosynthesis and related parameters | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.access | info:eu-repo/semantics/openAccess | es_ES |