Copper toxicity on the marine microalga Phaeodactylum tricornutum: effects on photosynthesis and related parameters
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Copper toxicity on the marine microalga Phaeodactylum tricornutum: effects on photosynthesis and related parametersData
1995-02Cita bibliográfica
Cid A, Herrero C, Torres E, Abalde J. Copper toxicity on the marine microalga phaeodactylum tricornutum: Effects on photosynthesis and related parameters. Aquatic Toxicology. 1995;31(2):165-74.
Resumo
Essential heavy metals, as copper, can be toxic for microalgae at high concentrations. Copper affected growth and other parameters closely related to photosynthesis of the marine diatom Phaeodactylum tricornutum. A copper concentration of 0.10 mg l−1 provoked about 50% growth reduction and 1 mg l−1inhibited the growth. Copper also interfered with photosynthesis and ATP production. A copper concentration of 0.5 mg l−1 reduced in a 50% the photosynthetic rate. Therefore, growth is more affected by copper than photosynthesis. Results of chlorophyll a fluorescence obtained by flow cytometry showed that copper's inhibitory effect on PS II activity is located on its oxidizing side. The lower copper concentration assayed provoked a significant decrease in the cellular pool of ATP. Pigment analysis by HPLC showed that copper affected the pigment pattern of P. tricornutum. Important changes were observed for chlorophyll a and its allomer: chlorophyll a proportion decreased while its allomer increased with the copper concentration, being maximum at 1 mg Cu l−1. The study of the intracellular pH by flow cytometry revealed that P. tricornutumcells exposed to 0.5 and 1 mg Cu l−1 showed an intracellular pH higher than control cultures cells, explaining the high proportion of the chlorophyll a allomer in these cells.
Palabras chave
Copper toxicity
Phaeodactylum tricornutum
Photosynthesis
Flow cytometry
Phaeodactylum tricornutum
Photosynthesis
Flow cytometry