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A novel procedure for protein extraction from formalin-fixed paraffin-embedded tissues
(Wiley, 2011-05-18)
[Abstract] Most of the archived pathological specimens in hospitals are kept as formalin-fixed paraffin-embedded tissues (FFPE) for long-term preservation. Up to now, these samples are only used for immunohistochemistry ...
Type 1 Diabetes Mellitus reversal via implantation of magnetically purified microencapsulated pseudoislets
(Elsevier, 2019-02-10)
[Abstract] Microencapsulation of pancreatic islets for the treatment of Type I Diabetes Mellitus (T1DM) generates a high quantity of empty microcapsules, resulting in high therapeutic graft volumes that can enhance the ...
Wnt and RUNX2 mediate cartilage breakdown by osteoarthritis synovial fibroblast‐derived ADAMTS‐7 and ‐12
(Wiley, 2019-03-22)
[Abstract] Failure of therapeutic approaches for the treatment of osteoarthritis (OA) based on the inhibition of metalloproteinases, might be because of their constitutive expression in homeostasis, together with their ...
Mitochondrial activity is modulated by TNFα and IL-1β in normal human chondrocyte cells
(Elsevier, 2006-05-05)
[Abstract] Objective. Pro-inflammatory cytokines play an important role in osteoarthritis (OA). In osteoarthritic cartilage, chondrocytes exhibit an alteration in mitochondrial activity. This study analyzes the effect of ...
Leukocyte telomere length in patients with radiographic knee osteoarthritis
(Wiley, 2019-11-29)
[Abstract] Relative mean telomere sequence amount was determined by quantitative PCR (qPCR) of peripheral blood leukocyte (PBL) samples obtained at recruitment (n = 310) from individuals from the Osteoarthritis (OA) ...
Generating Rho-0 cells using mesenchymal stem cell lines
(PLoS, 2016-10-20)
[Abstract] Introduction, The generation of Rho-0 cells requires the use of an immortalization process, or tumor cell selection, followed by culture in the presence of ethidium bromide (EtBr), incurring the drawbacks its ...
Evaluation of 12 GWAS-drawn SNPs as biomarkers of rheumatoid arthritis response to TNF inhibitors. A potential SNP association with response to etanercept
(PLoS, 2019-02-28)
[Abstract] Research in rheumatoid arthritis (RA) is increasingly focused on the discovery of biomarkers that could enable personalized treatments. The genetic biomarkers associated with the response to TNF inhibitors (TNFi) ...
Hif-1α knockdown reduces glycolytic metabolism and induces cell death of human synovial fibroblasts under normoxic conditions
(Nature, 2017-06-16)
[Abstract] Increased glycolysis and HIF-1α activity are characteristics of cells under hypoxic or inflammatory conditions. Besides, in normal O2 environments, elevated rates of glycolysis support critical cellular mechanisms ...
Discovery of circulating proteins associated to knee radiographic osteoarthritis
(Nature, 2017-03-09)
[Abstract] Currently there are no sufficiently sensitive biomarkers able to reflect changes in joint remodelling during osteoarthritis (OA). In this work, we took an affinity proteomic approach to profile serum samples for ...