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dc.contributor.authorGubern-Mérida, Carme
dc.contributor.authorComajoan, Pau
dc.contributor.authorHuguet, Gemma
dc.contributor.authorGarcía-Yebenes, Isaac
dc.contributor.authorLizasoain, Ignacio
dc.contributor.authorMoro, María Ángeles
dc.contributor.authorPuig-Parnau, Irene
dc.contributor.authorSánchez, Juan Manuel
dc.contributor.authorSerena, Joaquín
dc.contributor.authorKádár, Elisabet
dc.contributor.authorCastellanos, María del Mar
dc.date.accessioned2024-11-06T08:35:12Z
dc.date.available2024-11-06T08:35:12Z
dc.date.issued2022-01-05
dc.identifier.citationGubern-Mérida C, Comajoan P, Huguet G, García-Yebenes I, Lizasoain I, Moro MA, Puig-Parnau I, Sánchez JM, Serena J, Kádár E, Castellanos M. Cav-1 protein levels in serum and infarcted brain correlate with hemorrhagic volume in a mouse model of thromboembolic stroke, independently of rt-PA administration. Mol Neurobiol. 2022 Feb;59(2):1320-1332.es_ES
dc.identifier.issn0893-7648
dc.identifier.urihttp://hdl.handle.net/2183/39950
dc.description.abstract[Abstract] Thrombolytic therapy with recombinant tissue plasminogen activator (rt-PA) is currently the only FDA-approved drug for acute ischemic stroke. However, its administration is still limited due to the associated increased risk of hemorrhagic transformation (HT). rt-PA may exacerbate blood-brain barrier (BBB) injury by several mechanisms that have not been fully elucidated. Caveolin-1 (Cav-1), a major structural protein of caveolae, has been linked to the endothelial barrier function. The effects of rt-PA on Cav-1 expression remain largely unknown. Here, Cav-1 protein expression after ischemic conditions, with or without rt-PA administration, was analyzed in a murine thromboembolic middle cerebral artery occlusion (MCAO) and in brain microvascular endothelial bEnd.3 cells subjected to oxygen/glucose deprivation (OGD). Our results show that Cav-1 is overexpressed in endothelial cells of infarcted area and in bEnd.3 cell line after ischemia but there is disagreement regarding rt-PA effects on Cav-1 expression between both experimental models. Delayed rt-PA administration significantly reduced Cav-1 total levels from 24 to 72 h after reoxygenation and increased pCav-1/Cav-1 at 72 h in the bEnd.3 cells while it did not modify Cav-1 immunoreactivity in the infarcted area at 24 h post-MCAO. Importantly, tissue Cav-1 positively correlated with Cav-1 serum levels at 24 h post-MCAO and negatively correlated with the volume of hemorrhage after infarction, the latter supporting a protective role of Cav-1 in cerebral ischemia. In addition, the negative association between baseline serum Cav-1 levels and hemorrhagic volume points to a potential usefulness of baseline serum Cav-1 levels to predict hemorrhagic volume, independently of rt-PA administration.es_ES
dc.description.sponsorshipThis work was supported by grants from Instituto de Salud Carlos III and co-financed by the European Development Regional Fund “A Way to Achieve Europe” Health Strategic Action Program PI13/02258 and PI17/02123 (MC), PI20/00535 (IL), and Spanish Stroke Research Network RETICS RD12/0014/0010 (MC), and RD16/0019/0003 (JS), RD16/0019/0004 (MC), and RD16/0019/0009 (IL); from Regional Madrid Government B2017/BMD- 3688 (IL); from Spanish Ministry of Science and Innovation PID2019-106581RB-I00 (MAM); from Leducq Foundation for Cardiovascular Research TNE-19CVD01 (MAM); and from Fundación La Caixa HR17_00527 (MAM). P. Comajoan was a recipient of a predoctoral fellowship from the University of Girona (IF-UdG 2015).es_ES
dc.description.sponsorshipinfo:eu-repo/grantAgreement/MINECO/Programa Estatal de I+D+I Orientada a los Retos de la Sociedad/PI13%2F02258/ES/Transformación hemorrágica en isquemia cerebral tras administración de rt-PA: Participación de biomarcadores endoteliales y endotelio-protección mediante administración de péptidoses_ES
dc.description.sponsorshipinfo:eu-repo/grantAgreement/ISCIII/Programa Estatal de Fomento de la Investigación Científica y Técnica de Excelencia/PI17%2F02123/ES/DETERMINACION DE MIRNAS CIRCULANTES COMO BIOMARCADORES PREDICTIVOS DE TRANSFORMACION HEMORRAGICA EN ISQUEMIA CEREBRAL TRAS ADMINISTRACION DE RT-PA: ESTUDIO CLINICO Y PRE-CLINICO.es_ES
dc.description.sponsorshipinfo:eu-repo/grantAgreement/ISCIII/Programa Estatal de Generación de Conocimiento y Fortalecimiento del Sistema Español de I+D+I/PI20%2F00535/ES/NETS E ICTUS: INMUNOTROMBOSIS, ENVEJECIMIENTO E INFECCION. (NETSTROKE)es_ES
dc.description.sponsorshipinfo:eu-repo/grantAgreement/MINECO/Acción Estratégica de Salud/RD12%2F0014%2F0010/ES/Enfermedades vasculares cerebrales (Ictus)es_ES
dc.description.sponsorshipinfo:eu-repo/grantAgreement/MINECO/Programa Estatal de I+D+I Orientada a los Retos de la Sociedad/RD16%2F0019%2F0003/ES/Red de Enfermedades Vasculares Cerebrales. INVICTUS PLUSes_ES
dc.description.sponsorshipinfo:eu-repo/grantAgreement/MINECO/Programa Estatal de I+D+I Orientada a los Retos de la Sociedad/RD16%2F0019%2F0004/ES/Red de Enfermedades Vasculares Cerebrales. INVICTUS PLUSes_ES
dc.description.sponsorshipinfo:eu-repo/grantAgreement/MINECO/Programa Estatal de I+D+I Orientada a los Retos de la Sociedad/RD16%2F0019%2F0009/ES/Red de Enfermedades Vasculares Cerebrales. INVICTUS PLUSes_ES
dc.description.sponsorshipComunidad de Madrid; B2017/BMD-3688es_ES
dc.description.sponsorshipinfo:eu-repo/grantAgreement/AEI/Programa Estatal de Generación de Conocimiento y Fortalecimiento Científico y Tecnológico del Sistema de I+D+i/PID2019-106581RB-I00/ES/PAPEL DE ALTERACIONES DE LA NEUROGENESIS HIPOCAMPAL ADULTA EN EL DETERIORO COGNITIVO Y LA DEMENCIA VASCULARESes_ES
dc.description.sponsorshipLeducq Foundation for Cardiovascular Research; TNE-19CVD01es_ES
dc.description.sponsorshipFundación La Caixa; HR17_00527es_ES
dc.description.sponsorshipUniversitat de Girona; IF-UdG 2015es_ES
dc.language.isoenges_ES
dc.publisherSpringer Naturees_ES
dc.relation.urihttps://doi.org/10.1007/s12035-021-02644-yes_ES
dc.rightsThis version of the article has been accepted for publication, after peer review and is subject to Springer Nature’s AM terms of use, but is not the Version of Record and does not reflect post-acceptance improvements, or any corrections. The Version of Record is available online at Springer Link.es_ES
dc.subjectBlood-brain barrieres_ES
dc.subjectCaveolin-1es_ES
dc.subjectMiddle cerebral artery occlusiones_ES
dc.subjectOxygen/glucose deprivationes_ES
dc.subjectRecombinant tissue plasminogen activatores_ES
dc.subjectStrokees_ES
dc.titleCav-1 protein levels in serum and infarcted brain correlate with hemorrhagic volume in a mouse model of thromboembolic stroke, independently of rt-PA administrationes_ES
dc.typejournal articlees_ES
dc.rights.accessRightsopen accesses_ES
UDC.journalTitleMolecular Neurobiologyes_ES
UDC.volume59es_ES
UDC.startPage1320es_ES
UDC.endPage1332es_ES
dc.identifier.doi10.1007/s12035-021-02644-y
UDC.coleccionInvestigaciónes_ES
UDC.grupoInvEnfermidades Cerebrovasculares: Neuroloxía Clínica e Traslacional (INIBIC)es_ES
UDC.institutoCentroINIBIC - Instituto de Investigacións Biomédicas de A Coruñaes_ES


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