dc.contributor.author | Gubern-Mérida, Carme | |
dc.contributor.author | Comajoan, Pau | |
dc.contributor.author | Huguet, Gemma | |
dc.contributor.author | García-Yebenes, Isaac | |
dc.contributor.author | Lizasoain, Ignacio | |
dc.contributor.author | Moro, María Ángeles | |
dc.contributor.author | Puig-Parnau, Irene | |
dc.contributor.author | Sánchez, Juan Manuel | |
dc.contributor.author | Serena, Joaquín | |
dc.contributor.author | Kádár, Elisabet | |
dc.contributor.author | Castellanos, María del Mar | |
dc.date.accessioned | 2024-11-06T08:35:12Z | |
dc.date.available | 2024-11-06T08:35:12Z | |
dc.date.issued | 2022-01-05 | |
dc.identifier.citation | Gubern-Mérida C, Comajoan P, Huguet G, García-Yebenes I, Lizasoain I, Moro MA, Puig-Parnau I, Sánchez JM, Serena J, Kádár E, Castellanos M. Cav-1 protein levels in serum and infarcted brain correlate with hemorrhagic volume in a mouse model of thromboembolic stroke, independently of rt-PA administration. Mol Neurobiol. 2022 Feb;59(2):1320-1332. | es_ES |
dc.identifier.issn | 0893-7648 | |
dc.identifier.uri | http://hdl.handle.net/2183/39950 | |
dc.description.abstract | [Abstract] Thrombolytic therapy with recombinant tissue plasminogen activator (rt-PA) is currently the only FDA-approved drug for acute ischemic stroke. However, its administration is still limited due to the associated increased risk of hemorrhagic transformation (HT). rt-PA may exacerbate blood-brain barrier (BBB) injury by several mechanisms that have not been fully elucidated. Caveolin-1 (Cav-1), a major structural protein of caveolae, has been linked to the endothelial barrier function. The effects of rt-PA on Cav-1 expression remain largely unknown. Here, Cav-1 protein expression after ischemic conditions, with or without rt-PA administration, was analyzed in a murine thromboembolic middle cerebral artery occlusion (MCAO) and in brain microvascular endothelial bEnd.3 cells subjected to oxygen/glucose deprivation (OGD). Our results show that Cav-1 is overexpressed in endothelial cells of infarcted area and in bEnd.3 cell line after ischemia but there is disagreement regarding rt-PA effects on Cav-1 expression between both experimental models. Delayed rt-PA administration significantly reduced Cav-1 total levels from 24 to 72 h after reoxygenation and increased pCav-1/Cav-1 at 72 h in the bEnd.3 cells while it did not modify Cav-1 immunoreactivity in the infarcted area at 24 h post-MCAO. Importantly, tissue Cav-1 positively correlated with Cav-1 serum levels at 24 h post-MCAO and negatively correlated with the volume of hemorrhage after infarction, the latter supporting a protective role of Cav-1 in cerebral ischemia. In addition, the negative association between baseline serum Cav-1 levels and hemorrhagic volume points to a potential usefulness of baseline serum Cav-1 levels to predict hemorrhagic volume, independently of rt-PA administration. | es_ES |
dc.description.sponsorship | This work was supported by grants from Instituto de Salud Carlos III and co-financed by the European Development Regional Fund “A Way to Achieve Europe” Health Strategic Action Program PI13/02258 and PI17/02123 (MC), PI20/00535 (IL), and Spanish Stroke Research Network RETICS RD12/0014/0010 (MC), and RD16/0019/0003 (JS), RD16/0019/0004 (MC), and RD16/0019/0009 (IL); from Regional Madrid Government B2017/BMD- 3688 (IL); from Spanish Ministry of Science and Innovation PID2019-106581RB-I00 (MAM); from Leducq Foundation for Cardiovascular Research TNE-19CVD01 (MAM); and from Fundación La Caixa HR17_00527 (MAM). P. Comajoan was a recipient of a predoctoral fellowship from the University of Girona (IF-UdG 2015). | es_ES |
dc.description.sponsorship | info:eu-repo/grantAgreement/MINECO/Programa Estatal de I+D+I Orientada a los Retos de la Sociedad/PI13%2F02258/ES/Transformación hemorrágica en isquemia cerebral tras administración de rt-PA: Participación de biomarcadores endoteliales y endotelio-protección mediante administración de péptidos | es_ES |
dc.description.sponsorship | info:eu-repo/grantAgreement/ISCIII/Programa Estatal de Fomento de la Investigación Científica y Técnica de Excelencia/PI17%2F02123/ES/DETERMINACION DE MIRNAS CIRCULANTES COMO BIOMARCADORES PREDICTIVOS DE TRANSFORMACION HEMORRAGICA EN ISQUEMIA CEREBRAL TRAS ADMINISTRACION DE RT-PA: ESTUDIO CLINICO Y PRE-CLINICO. | es_ES |
dc.description.sponsorship | info:eu-repo/grantAgreement/ISCIII/Programa Estatal de Generación de Conocimiento y Fortalecimiento del Sistema Español de I+D+I/PI20%2F00535/ES/NETS E ICTUS: INMUNOTROMBOSIS, ENVEJECIMIENTO E INFECCION. (NETSTROKE) | es_ES |
dc.description.sponsorship | info:eu-repo/grantAgreement/MINECO/Acción Estratégica de Salud/RD12%2F0014%2F0010/ES/Enfermedades vasculares cerebrales (Ictus) | es_ES |
dc.description.sponsorship | info:eu-repo/grantAgreement/MINECO/Programa Estatal de I+D+I Orientada a los Retos de la Sociedad/RD16%2F0019%2F0003/ES/Red de Enfermedades Vasculares Cerebrales. INVICTUS PLUS | es_ES |
dc.description.sponsorship | info:eu-repo/grantAgreement/MINECO/Programa Estatal de I+D+I Orientada a los Retos de la Sociedad/RD16%2F0019%2F0004/ES/Red de Enfermedades Vasculares Cerebrales. INVICTUS PLUS | es_ES |
dc.description.sponsorship | info:eu-repo/grantAgreement/MINECO/Programa Estatal de I+D+I Orientada a los Retos de la Sociedad/RD16%2F0019%2F0009/ES/Red de Enfermedades Vasculares Cerebrales. INVICTUS PLUS | es_ES |
dc.description.sponsorship | Comunidad de Madrid; B2017/BMD-3688 | es_ES |
dc.description.sponsorship | info:eu-repo/grantAgreement/AEI/Programa Estatal de Generación de Conocimiento y Fortalecimiento Científico y Tecnológico del Sistema de I+D+i/PID2019-106581RB-I00/ES/PAPEL DE ALTERACIONES DE LA NEUROGENESIS HIPOCAMPAL ADULTA EN EL DETERIORO COGNITIVO Y LA DEMENCIA VASCULARES | es_ES |
dc.description.sponsorship | Leducq Foundation for Cardiovascular Research; TNE-19CVD01 | es_ES |
dc.description.sponsorship | Fundación La Caixa; HR17_00527 | es_ES |
dc.description.sponsorship | Universitat de Girona; IF-UdG 2015 | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Springer Nature | es_ES |
dc.relation.uri | https://doi.org/10.1007/s12035-021-02644-y | es_ES |
dc.rights | This version of the article has been accepted for publication, after peer review and is subject to Springer Nature’s AM terms of use, but is not the Version of Record and does not reflect post-acceptance improvements, or any corrections. The Version of Record is available online at Springer Link. | es_ES |
dc.subject | Blood-brain barrier | es_ES |
dc.subject | Caveolin-1 | es_ES |
dc.subject | Middle cerebral artery occlusion | es_ES |
dc.subject | Oxygen/glucose deprivation | es_ES |
dc.subject | Recombinant tissue plasminogen activator | es_ES |
dc.subject | Stroke | es_ES |
dc.title | Cav-1 protein levels in serum and infarcted brain correlate with hemorrhagic volume in a mouse model of thromboembolic stroke, independently of rt-PA administration | es_ES |
dc.type | journal article | es_ES |
dc.rights.accessRights | open access | es_ES |
UDC.journalTitle | Molecular Neurobiology | es_ES |
UDC.volume | 59 | es_ES |
UDC.startPage | 1320 | es_ES |
UDC.endPage | 1332 | es_ES |
dc.identifier.doi | 10.1007/s12035-021-02644-y | |
UDC.coleccion | Investigación | es_ES |
UDC.grupoInv | Enfermidades Cerebrovasculares: Neuroloxía Clínica e Traslacional (INIBIC) | es_ES |
UDC.institutoCentro | INIBIC - Instituto de Investigacións Biomédicas de A Coruña | es_ES |