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The Lin28/Let-7 system in early human embryonic tissue and ectopic pregnancy
dc.contributor.author | Lozoya, Teresa | |
dc.contributor.author | Domínguez, Francisco | |
dc.contributor.author | Romero-Ruiz, Antonio | |
dc.contributor.author | Steffani, Liliana | |
dc.contributor.author | Martínez, Sebastián | |
dc.contributor.author | Monterde, Mercedes | |
dc.contributor.author | Ferri, Blanca | |
dc.contributor.author | Núñez, María José | |
dc.contributor.author | Romero-Espinós, Ainhoa | |
dc.contributor.author | Zamora, Omar | |
dc.contributor.author | Guerra, Marta | |
dc.contributor.author | Sangiao-Alvarellos, Susana | |
dc.contributor.author | Vega, Olivia | |
dc.contributor.author | Simón, Carlos | |
dc.contributor.author | Pellicer, Antonio | |
dc.contributor.author | Tena-Sempere, Manuel | |
dc.date.accessioned | 2024-08-26T08:57:13Z | |
dc.date.available | 2024-08-26T08:57:13Z | |
dc.date.issued | 2014-01-31 | |
dc.identifier.citation | Lozoya T, Domínguez F, Romero-Ruiz A, Steffani L, Martínez S, Monterde M, Ferri B, Núñez MJ, AinhoaRomero-Espinós, Zamora O, Gurrea M, Sangiao-Alvarellos S, Vega O, Simón C, Pellicer A, Tena-Sempere M. The Lin28/Let-7 system in early human embryonic tissue and ectopic pregnancy. PLoS One. 2014 Jan 31;9(1):e87698. | es_ES |
dc.identifier.issn | 1932-6203 | |
dc.identifier.uri | http://hdl.handle.net/2183/38688 | |
dc.description.abstract | [Abstract] Our objective was to determine the expression of the elements of the Lin28/Let-7 system, and related microRNAs (miRNAs), in early stages of human placentation and ectopic pregnancy, as a means to assess the potential role of this molecular hub in the pathogenesis of ectopic gestation. Seventeen patients suffering from tubal ectopic pregnancy (cases) and forty-three women with normal on-going gestation that desired voluntary termination of pregnancy (VTOP; controls) were recruited for the study. Embryonic tissues were subjected to RNA extraction and quantitative PCR analyses for LIN28B, Let-7a, miR-132, miR-145 and mir-323-3p were performed. Our results demonstrate that the expression of LIN28B mRNA was barely detectable in embryonic tissue from early stages of gestation and sharply increased thereafter to plateau between gestational weeks 7-9. In contrast, expression levels of Let-7, mir-132 and mir-145 were high in embryonic tissue from early gestations (≤ 6-weeks) and abruptly declined thereafter, especially for Let-7. Opposite trends were detected for mir-323-3p. Embryonic expression of LIN28B mRNA was higher in early stages (≤ 6-weeks) of ectopic pregnancy than in normal gestation. In contrast, Let-7a expression was significantly lower in early ectopic pregnancies, while miR-132 and miR-145 levels were not altered. Expression of mir-323-3p was also suppressed in ectopic embryonic tissue. We are the first to document reciprocal changes in the expression profiles of the gene encoding the RNA-binding protein, LIN28B, and the related miRNAs, Let-7a, mir-132 and mir-145, in early stages of human placentation. This finding suggests the potential involvement of LIN28B/Let-7 (de)regulated pathways in the pathophysiology of ectopic pregnancy in humans. | es_ES |
dc.description.sponsorship | This work was funded by grant BFU 2011-25021 from Ministerio de Economia y Competitividad, Spain (co-funded by FEDER Program from EU) and Project P08-CVI-03788 (Junta de Andalucía, Spain) (to MTS). CIBER Fisiopatología de la Obesidad y Nutrición is an initiative of Instituto de Salud Carlos III, Ministerio de Sanidad, Spain. The participation of FD in this work was supported by the Spanish Ministry of Economy and Competitiveness, through Miguel Servet Programme (CP13/00075). | es_ES |
dc.description.sponsorship | info:eu-repo/grantAgreement/MICINN/Programa Nacional de Investigación Fundamental/BFU2011-25021/ES/PUBERTAD - NUEVOS SISTEMAS DE REGULACION POR NEUROPEPTIDOS Y MICRORNAS, Y SU INTERACCION CON SEÑALES METABOLICAS Y OBESIDAD | es_ES |
dc.description.sponsorship | info:eu-repo/grantAgreement/Junta de Andalucía/Programa Nacional de Investigación Fundamental/P08-CVI-03788/ES/Investigación traslacional en pubertad e infertilidad: Análisis del sistema kiss-1/GPR54 | es_ES |
dc.description.sponsorship | info:eu-repo/grantAgreement/MINECO/Programa Estatal de I+D+I Orientada a los Retos de la Sociedad/CP13%2F00075/ES/ | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | PLoS One | es_ES |
dc.relation.uri | https://doi.org/10.1371/journal.pone.0087698 | es_ES |
dc.rights | Creative Commons Attribution 4.0 International License (CC-BY 4.0) | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es/ | * |
dc.subject | Embryo, Mammalian | es_ES |
dc.subject | Gene Expression Regulation | es_ES |
dc.subject | MicroRNAs | es_ES |
dc.subject | Placentation | es_ES |
dc.subject | Pregnancy, Ectopic | es_ES |
dc.subject | RNA-Binding Proteins | es_ES |
dc.title | The Lin28/Let-7 system in early human embryonic tissue and ectopic pregnancy | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.access | info:eu-repo/semantics/openAccess | es_ES |
UDC.volume | 9 | es_ES |
UDC.issue | 1 | es_ES |
UDC.startPage | e87698 | es_ES |
dc.identifier.doi | 10.1371/journal.pone.0087698 |
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