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Carotenoids production and extraction from Yarrowia lipolytica cells: A biocompatible approach using biosolvents
dc.contributor.author | Kennes, Christian | |
dc.contributor.author | Naveira-Pazos, Cecilia | |
dc.contributor.author | Veiga, María Carmen | |
dc.contributor.author | Mussagy, Cassamo U. | |
dc.contributor.author | Farias, Fabiane O. | |
dc.contributor.author | Pereira, Jorge F.B. | |
dc.date.accessioned | 2024-08-09T07:22:47Z | |
dc.date.available | 2024-08-09T07:22:47Z | |
dc.date.issued | 2024 | |
dc.identifier.citation | Naveira-Pazos, C., Veiga, M. C., Mussagy, C. U., Farias, F. O., Kennes, C., & Pereira, J. F. B. (2024). Carotenoids production and extraction from Yarrowia lipolytica cells: A biocompatible approach using biosolvents. Separation and Purification Technology, 343. https://doi.org/10.1016/J.SEPPUR.2024.127136 | es_ES |
dc.identifier.uri | http://hdl.handle.net/2183/38495 | |
dc.description.abstract | [Abstract] The use of carotenoids, pigments with significant importance in the food and pharmaceutical industries, has been widely recognized because of their functions as colorants and antioxidants. The production of carotenoids from oleaginous yeasts is an efficient and eco-friendly alternative to that of synthetic carotenoids. Yarrowia lipolytica, due to its ability to synthesize high-value-added compounds, including carotenoids and lipids, has emerged as a promising biotechnological platform for this purpose. This study aimed to evaluate the efficiency of biosolvents for carotenoid extraction with the goal of replacing toxic organic solvents that pose risks to both human health and the environment. The Solid-Liquid Extraction (SLE) technique was employed to disrupt the rigid cell wall and extract intracellular pigments. Subsequent extractions were conducted using a 2:3:1 (w/w/w) ratio of ethanol:ethyl acetate:water, resulting in a total β-carotene concentration of approximately 5 mg β-carotene equivalent/g dry biomass. The COSMO-SAC model was utilized to elucidate the solute–solvent affinity, and the results obtained were consistent with the experimental findings. To improve the process, various ratios of biosolvents were investigated, and a subsequent Solid-Liquid-Liquid Extraction (SLLE) was introduced. Process integration and adjustments to the mixed biosolvent composition achieved yields ranging from 50 to 100 % in just four consecutive extractions. The proposed integrative platform not only enhances the efficiency of the extraction process but also enables the fractionation and purification of bioproducts from Y. lipolytica cells, thereby maximizing their biotechnological potential. | es_ES |
dc.description.sponsorship | Cecilia Naveira Pazos thanks Inditex-UDC 2023 for financial support during her short-term visit to the University of Coimbra. Part of this study undertaken at University of La Coruña (UDC) was financed by the Spanish Ministry of Science and Innovation (PID2020-117805RB-I00 and TED2021-130055B-I00). The BIOENGIN group at UDC thanks Xunta de Galicia for financial support to Competitive Reference Research Groups (ED431C 2021/55). CERES is supported by the Fundação para a Ciência e Tecnologia (FCT) through UIDB/EQU/00102/2020 projects UIDP/EQU/00102/2020. CUM was supported by grants from VINCI-DI PUCV (grants 039.338/2023 and 039.369/2023). FOF is grateful to the Brazilian National Council for Scientific and Technological Development (405442/2023-8). | es_ES |
dc.description.sponsorship | Xunta de Galicia; ED431C 2021/55 | es_ES |
dc.description.sponsorship | Portugal. Fundação para a Ciência e Tecnologia; UIDB/EQU/00102/2020 | es_ES |
dc.description.sponsorship | Brasil. Conselho Nacional de Desenvolvimento Científico e Tecnológico; 405442/2023-8 | es_ES |
dc.description.sponsorship | Chile. Pontificia Universidad Católica de Valparaíso; 039.338/2023 | es_ES |
dc.description.sponsorship | Chile. Pontificia Universidad Católica de Valparaíso; 039.369/2023 | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Elsevier | es_ES |
dc.relation | info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-117805RB-I00/ES/PROCESOS DE BIOREFINERIA SOSTENIBLE PARA LA PRODUCCION DE BIOCOMBUSTIBLES, BIOPOLIMEROS Y OTROS BIOPRODUCTOS VIA ACIDOS CARBOXILICOS/ | es_ES |
dc.relation | info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/TED2021-130055B-I00/ES/PROCESOS DE DESCARBONIZACION INNOVADORES BASADOS EN LA ELIMINACION DE DIOXIDO DE CARBONO Y SU BIOCONVERSION EN BIOCOMBUSTIBLES Y BIOPRODUCTOS | es_ES |
dc.relation.uri | https://doi.org/10.1016/j.seppur.2024.127136 | es_ES |
dc.rights | CC BY-NC-ND 4.0 Attribution-NonCommercial-NoDerivatives 4.0 International | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/es/ | * |
dc.subject | Yarrowia lipolytica | es_ES |
dc.subject | Extraction | es_ES |
dc.subject | Carotenoids | es_ES |
dc.subject | β-carotene | es_ES |
dc.subject | Biosolvents | es_ES |
dc.title | Carotenoids production and extraction from Yarrowia lipolytica cells: A biocompatible approach using biosolvents | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.access | info:eu-repo/semantics/openAccess | es_ES |
UDC.journalTitle | Separation and Purification Technology | es_ES |
UDC.volume | 343 | es_ES |
UDC.startPage | Article 127136 | es_ES |
dc.identifier.doi | 10.1016/j.seppur.2024.127136 |
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