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dc.contributor.authorValdiglesias, Vanessa
dc.contributor.authorGiunta, Simona
dc.contributor.authorFenech, M.
dc.contributor.authorNeri, Monica
dc.contributor.authorBonassi, Stefano
dc.date.accessioned2024-04-22T14:11:19Z
dc.date.issued2013-02-13
dc.identifier.citationVanessa Valdiglesias, Simona Giunta, Michael Fenech, Monica Neri, Stefano Bonassi, γH2AX as a marker of DNA double strand breaks and genomic instability in human population studies, Mutation Research/Reviews in Mutation Research, Volume 753, Issue 1, 2013, Pages 24-40, ISSN 1383-5742, https://doi.org/10.1016/j.mrrev.2013.02.001. (https://www.sciencedirect.com/science/article/pii/S1383574213000239)es_ES
dc.identifier.issn1383-5742
dc.identifier.urihttp://hdl.handle.net/2183/36293
dc.description.abstract[Abstract] DNA double strand breaks (DSB) are the gravest form of DNA damage in eukaryotic cells. Failure to detect DSB and activate appropriate DNA damage responses can cause genomic instability, leading to tumorigenesis and possibly accelerated aging. Phosphorylated histone H2AX (γH2AX) is used as a biomarker of cellular response to DSB and its potential for monitoring DNA damage and repair in human populations has been explored in this review. A systematic search was conducted in PubMed for articles, in English, on human studies reporting γH2AX as a biomarker of either DNA repair or DNA damage. A total of 68 publications were identified. Thirty-four studies (50.0%) evaluated the effect of medical procedures or treatments on γH2AX levels; 20 (29.4%) monitored γH2AX in specific pathological conditions with a case/control or case/case design; 5 studies (7.4%) evaluated the effect of environmental genotoxic exposures, and 9 (13.2%) were descriptive studies on cancer and aging. Peripheral blood lymphocytes (44.6%) or biopsies/tissue specimens (24.3%) were the most commonly used samples. γH2AX was scored by optical microscopy as immunostained foci (78%), or by flow cytometry (16%). Critical features affecting the reliability of the assay, including protocols heterogeneity, specimen, cell cycle, kinetics, study design, and statistical analysis, are hereby discussed. Because of its sensitivity, efficiency and mechanistic relevance, the γH2AX assay has great potential as a DNA damage biomarker; however, the technical and epidemiological heterogeneity highlighted in this review infer a necessity for experimental standardization of the assay.es_ES
dc.description.sponsorshipThe study was supported by grants funded by the AIRC (Associazione Italiana per la Ricerca sul Cancro) [SB, VV], and INAIL (Istituto Nazionale Assicurazione contro gli Infortuni sul Lavoro) [SB, MN]. SG was supported by an International Cancer Technology Transfer (ICRETT) Fellowship from the Union for International Cancer Control (UICC).es_ES
dc.language.isoenges_ES
dc.publisherElsevieres_ES
dc.relation.urihttps://doi.org/10.1016/j.mrrev.2013.02.001es_ES
dc.rights© 2013 Elsevier B.V. All rights reserved.es_ES
dc.subjectγH2AXes_ES
dc.subjectDNA double strand breakses_ES
dc.subjectGenomic instabilityes_ES
dc.subjectHuman population studieses_ES
dc.subjectMolecular epidemiologyes_ES
dc.titleγH2AX as a Marker of DNA Double Strand Breaks and Genomic Instability in Human Population Studies [Review]es_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.rights.accessinfo:eu-repo/semantics/embargoedAccesses_ES
dc.date.embargoEndDate9999-99-99es_ES
dc.date.embargoLift10007-06-07
UDC.journalTitleMutation Research/Reviews in Mutation Researches_ES
UDC.volume753 (2013)es_ES
UDC.issue1es_ES
UDC.startPage24es_ES
UDC.endPage40es_ES
dc.identifier.doi10.1016/j.mrrev.2013.02.001


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