Effect of pH, Yeast Extract and Inorganic Carbon on Chain Elongation for Hexanoic Acid Production
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Effect of pH, Yeast Extract and Inorganic Carbon on Chain Elongation for Hexanoic Acid ProductionDate
2019-12-29Citation
Pau San-Valero, Haris Nalakath Abubackar, María C. Veiga, Christian Kennes, Effect of pH, yeast extract and inorganic carbon on chain elongation for hexanoic acid production, Bioresource Technology, Volume 300, 2020, 122659, ISSN 0960-8524, https://doi.org/10.1016/j.biortech.2019.122659. (https://www.sciencedirect.com/science/article/pii/S0960852419318887)
Abstract
[Abstract] Several anaerobic bioconversion technologies produce short chain volatile fatty acids and sometimes ethanol, which can together be elongated to hexanoic acid (C6 acid) by Clostridium kluyveri in a secondary fermentation process. Initiatives are needed to further optimize the process. Therefore, five strategies were tested aiming at elucidating their influence on hexanoic acid production from mixtures of acetic acid, butyric acid and ethanol. pH-regulated bioreactors, maintained at pH 7.5, 6.8 or 6.4 led to maximum C6 acid concentrations of, respectively, 19.4, 18.3 and 13.3 g L−1. At pH 6.8, yeast extract omission resulted in a decrease of the hexanoic acid concentration to 12.0 g L−1 while the addition of an inorganic carbon source, such as bicarbonate, for pH control, increased the C6 acid concentration up to 21.4 g L−1. This research provides guidelines for efficient improved production of hexanoic acid by pure cultures of C. kluyveri, contributing to the state of art.
Keywords
Caproic acid
Clostridium kluyveri
Chain elongation
Medium chain fatty acids
Clostridium kluyveri
Chain elongation
Medium chain fatty acids
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© 2019 Elsevier Ltd. All rights reserved.
ISSN
0960-8524