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dc.contributor.authorLaffon, Blanca
dc.contributor.authorValdiglesias, Vanessa
dc.contributor.authorPásaro, Eduardo
dc.contributor.authorMéndez, Josefina
dc.date.accessioned2024-01-09T20:22:11Z
dc.date.available2024-01-09T20:22:11Z
dc.date.issued2009-05-08
dc.identifier.citationLaffon, B., Valdiglesias, V., Pásaro, E. et al. The Organic Selenium Compound Selenomethionine Modulates Bleomycin-Induced DNA Damage and Repair in Human Leukocytes. Biol Trace Elem Res 133, 12–19 (2010). https://doi.org/10.1007/s12011-009-8407-9es_ES
dc.identifier.issn1559-0720
dc.identifier.urihttp://hdl.handle.net/2183/34784
dc.descriptionThis is an Accepted Version of the published document. This version of the article has been accepted for publication, after peer review (when applicable) and is subject to Springer Nature’s AM terms of use, but is not the Version of Record and does not reflect post-acceptance improvements, or any corrections. The Version of Record is available online at: https://doi.org/10.1007/s12011-009-8407-9es_ES
dc.description.abstract[Abstract] The objective of this work was to evaluate the effects of selenomethionine (SeMet) on the induction, repair, and persistence of DNA damage in human leukocytes challenged with bleomycin (BLM). Comet assay was used to determine DNA strand breaks and hOGG1 for the specific recognition of oxidative damage. Leukocytes were (A) stimulated with phytohemagglutinin, (B) damaged with BLM, and (C) incubated to allow DNA repair. Comet assay was performed after each phase. SeMet (50 μM) was supplemented either during phase A, B, or C, or AB, or ABC. Treatment with SeMet decreased BLM-induced stand breaks when added during phase AB. Results obtained after the repair period indicate that SeMet favors repair of DNA damage especially when applied during phase AB. The comparison between DNA damage before and after repair showed that BLM-induced damage was repaired better in the presence of SeMet. Our results showed antigenotoxic effect of SeMet on BLM-induced DNA and also on repair and persistence of this damage when applied before and simultaneously with BLM.es_ES
dc.description.sponsorshipThis work was funded by a grant from the Xunta de Galicia (INCITE08PXIB106155PR). V. Valdiglesias was supported by a fellowship from the University of A Coruñaes_ES
dc.description.sponsorshipGalicia. Xunta; INCITE08PXIB106155PRes_ES
dc.language.isoenges_ES
dc.publisherSpringeres_ES
dc.relation.urihttps://doi.org/10.1007/s12011-009-8407-9es_ES
dc.rights© Humana Press Inc. Subject to Springer Nature’s AM terms of use.es_ES
dc.subjectSelenomethioninees_ES
dc.subjectBleomycines_ES
dc.subjectComet assayes_ES
dc.subjectOxidative damagees_ES
dc.subjectAntigenotoxicityes_ES
dc.titleThe Organic Selenium Compound Selenomethionine Modulates Bleomycin-Induced DNA Damage and Repair in Human Leukocyteses_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.rights.accessinfo:eu-repo/semantics/openAccesses_ES
UDC.journalTitleBiological Trace Element Researches_ES
UDC.volume133 (2010)es_ES
UDC.startPage12es_ES
UDC.endPage19es_ES
dc.identifier.doi10.1007/s12011-009-8407-9


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