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dc.contributor.authorRamos, Daniel R.
dc.contributor.authorFernández-Pérez, María-Isabel
dc.contributor.authorFurtmüller, Paul G.
dc.contributor.authorObinger, Christian
dc.contributor.authorGarcía-Dopico, María-Victoria
dc.contributor.authorSantaballa, J. Arturo
dc.contributor.authorCanle, Moisés
dc.date.accessioned2022-03-17T12:26:54Z
dc.date.available2022-03-17T12:26:54Z
dc.date.issued2021-11-27
dc.identifier.citationD.R. Ramos, M.I. Fernández, P.G. Furtmüller, C. Obinger, M.V. García, J.A. Santaballa, M. Canle, Effect of ionizing radiation on human myeloperoxidase: Reaction with hydrated electrons, Journal of Photochemistry and Photobiology B: Biology. 226 (2022) 112369. https://doi.org/10.1016/j.jphotobiol.2021.112369es_ES
dc.identifier.issn1011-1344
dc.identifier.urihttp://hdl.handle.net/2183/30045
dc.descriptionFinanciado para publicación en acceso aberto: Universidade da Coruña/CISUGes_ES
dc.description.abstract[Abstract] Myeloperoxidase (MPO) is a myeloid-lineage restricted enzyme largely expressed in the azurophilic granules of neutrophils. It catalyses the formation of reactive oxygen species, mainly hypochlorous acid, contributing to anti-pathogenic defense. Disorders in the production or regulation of MPO may lead to a variety of health conditions, mainly of inflammatory origin, including autoimmune inflammation. We have studied the effect of ionizing radiation on the activity of MPO, as measured by the capacity retained by the enzyme to produce hypochlorous acid as reactive oxygen species after exposure to successive doses of solvated electrons, the strongest possible one-e− reducing agent in water. Chlorination activity was still present after a very high irradiation dose, indicating that radiation damage does not take place at the active site, hindered in the core of MPO structure. Decay kinetics show a dependence on the wavelength, supporting that the process must occur at peripheral functional groups situated on external and readily accessible locations of the enzyme. These results are relevant to understand the mechanism of resistance of our innate anti-pathogenic defense system and also to get insight into potential strategies to regulate MPO levels as a therapeutic target in autoimmune diseases.es_ES
dc.description.sponsorshipThis work was supported by: the Spanish Ministerio de Ciencia y Tecnología (CTQ2004-00534/BQU), the European Commission through the Access to Large-Scale Scientific Facilities Program (ref 41365), and the regional government of the Xunta de Galicia (Project GPC ED431B 2020/52)es_ES
dc.description.sponsorshipXunta de Galicia; ED431B 2020/52es_ES
dc.language.isoenges_ES
dc.publisherElsevieres_ES
dc.relationinfo:eu-repo/grantAgreement//Plan Nacional de I+D+i 2004-2007/CTQ2004-00534%2FBQU/ES/
dc.relation.urihttps://doi.org/10.1016/j.jphotobiol.2021.112369es_ES
dc.rightsAtribución-NoComercial-SinDerivadas 4.0 Internacionales_ES
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectMyeloperoxidasees_ES
dc.subjectPulse radiolysises_ES
dc.subjectChlorinating activityes_ES
dc.subjectOxidative stresses_ES
dc.subjectInflammationes_ES
dc.titleEffect of Ionizing Radiation on Human Myeloperoxidase: Reaction With Hydrated Electronses_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.rights.accessinfo:eu-repo/semantics/openAccesses_ES
UDC.journalTitleJournal of Photochemistry and Photobiology B: Biologyes_ES
UDC.volume226es_ES
UDC.startPage112369es_ES
dc.identifier.doi10.1016/j.jphotobiol.2021.112369


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