Influence of feedstock mix ratio on microbial dynamics during acidogenic fermentation for polyhydroxyalkanoates production

Abstract

[Abstract] The nature of microbial populations plays an essential role in the production of volatile fatty acids (VFA) during acidogenesis, the first stage in polyhydroxyalkanoates (PHA) production using mixed cultures. However, the composition of microbial communities is generally affected by substrate alterations. This work aimed to unravel the microbial dynamics in response to a gradual change in the feedstock composition in an acidogenic reactor, with subsequent PHA production. To achieve this, co-digestion of cheese whey and brewery wastewater (BW) was carried out for the production of VFA, in which the ratio of these feedstocks was varied by gradually increasing the proportion of BW from 0 up to 50% of the organic content. Bacteria such as Megasphaera, Bifidobacterium or Caproiciproducens were the most abundant in the first stages of the co-digestion. However, when BW reached 25% of the organic load, new taxa emerged and displaced the former ones; like Selenomonas, Ethanoligenens or an undefined member of the Bacteroidales order. Accordingly, the production of butyric acid dropped from 52 down to 27%, while the production of acetic acid increased from 36 up to 52%. Furthermore, the gradual increase of the BW ratio led to a progressive drop in the degree of acidification, from 72 down to 57%. In a subsequent approach, the VFA-rich streams, obtained from the co-digestion, were used as substrates in PHA accumulation tests. All the tests yielded similar PHA contents, but with slightly different monomeric composition. The overall results confirmed that the microbiome was altered by a gradual change in the feedstock composition and, consequently, the VFA profile and the monomeric composition of the biopolymer also did.