Development of a Fast and Efficient Method to Analyze Microplastics in Planktonic Samples
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http://hdl.handle.net/2183/30021
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- GI-QANAP - Artigos [30]
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Development of a Fast and Efficient Method to Analyze Microplastics in Planktonic SamplesAutor(es)
Data
2021-04-22Cita bibliográfica
López-Rosales, A., Andrade, J.M., Grueiro-Noche, G., Fernández-González, V., López-Mahía, P., Muniategui-Lorenzo, S., 2021. Development of a fast and efficient method to analyze microplastics in planktonic samples. Marine Pollution Bulletin 168, 112379. https://doi.org/10.1016/j.marpolbul.2021.112379
Resumo
[Abstract] Microplastics (MPs) affect plankton (a basis of the trophic chain) and planktivorous fish can ingest them through food confusion or by trophic transmission. Consensus to determine MPs in plankton is lacking and, so, three digestion treatments were evaluated: Alkaline (potassium hydroxide) and enzymatic (protease plus lipase) digestions, both combined with a hydrogen peroxide stage; and an oxidative method using a surfactant (sodium dodecyl sulfate) plus hydrogen peroxide. The alkaline method using potassium hydroxide was found to damage polystyrene. MPs were identified with a stereomicroscope and characterized by reflectance infrared microscopy in semi-automatic mode (using dedicated multi-well aluminium plates). Analytical recoveries for polypropylene, polystyrene, polyethylene, polyamide, polyvinyl chloride and polyethylene terephthalate were higher than 75%, 82% and 83% for the alkaline, enzymatic and oxidative treatments, respectively. The enzymatic method was successfully validated in a European interlaboratory exercise and the oxidative method was demonstrated to be a reliable, fast and cheaper alternative.
Palabras chave
Plankton
Microplastics
Infrared microscopy
Oxidative digestion
Alkaline digestion
Enzymatic digestion
Microplastics
Infrared microscopy
Oxidative digestion
Alkaline digestion
Enzymatic digestion
Descrición
Financiado para publicación en acceso aberto: Universidade da Coruña/CISUG
Versión do editor
Dereitos
Atribución 4.0 Internacional
ISSN
0025-326X