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dc.contributor.authorValdiglesias, Vanessa
dc.contributor.authorFernández-Bertólez, Natalia
dc.contributor.authorLema-Arranz, Carlota
dc.contributor.authorRodríguez-Fernández, Raquel
dc.contributor.authorPásaro, Eduardo
dc.contributor.authorReis, Ana Teresa
dc.contributor.authorTeixeira, João
dc.contributor.authorCosta, C.
dc.contributor.authorLaffon, Blanca
dc.date.accessioned2021-10-20T13:10:17Z
dc.date.available2021-10-20T13:10:17Z
dc.date.issued2021-07-27
dc.identifier.citationValdiglesias, V.; Fernández-Bertólez, N.; Lema-Arranz, C.; Rodríguez-Fernández, R.; Pásaro, E.; Reis, A.T.; Teixeira, J.P.; Costa, C.; Laffon, B. Salivary Leucocytes as In Vitro Model to Evaluate Nanoparticle-Induced DNA Damage. Nanomaterials 2021, 11, 1930. https://doi.org/10.3390/nano11081930es_ES
dc.identifier.issn2079-4991
dc.identifier.urihttp://hdl.handle.net/2183/28683
dc.description.abstract[Abstract] Metal oxide nanoparticles (NPs) have a wide variety of applications in many consumer products and biomedical practices. As a result, human exposure to these nanomaterials is highly frequent, becoming an issue of concern to public health. Recently, human salivary leucocytes have been proposed as an adequate non-invasive alternative to peripheral blood leucocytes to evaluate genotoxicity in vitro. The present study focused on proving the suitability of salivary leucocytes as a biomatrix in the comet assay for in vitro nanogenotoxicity studies, by testing some of the metal oxide NPs most frequently present in consumer products, namely, titanium dioxide (TiO₂), zinc oxide (ZnO), and cerium dioxide (CeO₂) NPs. Primary and oxidative DNA damage were evaluated by alkaline and hOGG1-modified comet assay, respectively. Any possible interference of the NPs with the methodological procedure or the hOGG1 activity was addressed before performing genotoxicity evaluation. Results obtained showed an increase of both primary and oxidative damage after NPs treatments. These data support the use of salivary leucocytes as a proper and sensitive biological sample for in vitro nanogenotoxicity studies, and contribute to increase the knowledge on the impact of metal oxide NPs on human health, reinforcing the need for a specific regulation of the nanomaterials use.es_ES
dc.description.sponsorshipThis research was funded by Xunta de Galicia [ED431B 2019/02]; Ministerio de Ciencia e Innovación (PID2020-114908GA-I00); Ministerio de Educación, Cultura y Deporte [BEAGAL18/00142, to V.V.]; CA17140 NANO2CLINIC COST Action; NanoBioBarriers (PTDC/MED-TOX/31162/2017) funded by Operational Program for Competitiveness and Internationalisation through European Regional Development Funds (FEDER/FNR) and through national funds by the Portuguese Foundation for Science and Technology (FCT). NanoLegaTox (POCI-01-0145-FEDER-029651) funded by Operational Program for Competitiveness and Internationalisation (COMPETE 2020) through FEDER, and through national funds by the Portuguese Foundation for Science and Technology (FCT). A.T. Reis funded by FCT Grant SFRH/BPD/122112/2016es_ES
dc.description.sponsorshipXunta de Galicia; ED431B 2019/02es_ES
dc.description.sponsorshipEuropean Cooperation in Science and Technology; CA17140es_ES
dc.description.sponsorshipPortugal. Fundação para a Ciência e a Tecnologia; PTDC/MED-TOX/31162/2017es_ES
dc.description.sponsorshipPortugal. Fundação para a Ciência e a Tecnologia; POCI-01-0145-FEDER-029651es_ES
dc.description.sponsorshipPortugal. Fundação para a Ciência e a Tecnologia; SFRH/BPD/122112/2016es_ES
dc.language.isoenges_ES
dc.publisherMDPIes_ES
dc.relationinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-114908GA-I00/ES/EVALUACION DEL RIESGO ASOCIADO A LA EXPOSICION A NANOMATERIALES: ESTRATEGIAS TOXICOLOGICAS IN VITRO, IN VIVO E IN SILICO/
dc.relationinfo:eu-repo/grantAgreement/MICINN/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/BEAGAL18%2F00142/ES/
dc.relation.urihttps://doi.org/10.3390/nano11081930es_ES
dc.rightsAtribución 4.0 Internacionales_ES
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectTiO₂ nanoparticleses_ES
dc.subjectZnO nanoparticleses_ES
dc.subjectCeO₂ nanoparticleses_ES
dc.subjectSalivary leukocyteses_ES
dc.subjectComet assayes_ES
dc.subjectInterferencees_ES
dc.subjectGenotoxicityes_ES
dc.titleSalivary Leucocytes as In Vitro Model to Evaluate Nanoparticle-Induced DNA Damagees_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.rights.accessinfo:eu-repo/semantics/openAccesses_ES
UDC.journalTitleNanomaterialses_ES
UDC.volume11es_ES
UDC.issue8es_ES
UDC.startPage1930es_ES


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