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dc.contributor.authorPlohl, Miroslav
dc.contributor.authorPrats, Eva
dc.contributor.authorMartínez-Lage, Andrés
dc.contributor.authorGonzález-Tizón, Ana M.
dc.contributor.authorMéndez, Josefina
dc.contributor.authorCornudella, Luis
dc.date.accessioned2020-06-26T17:54:59Z
dc.date.available2020-06-26T17:54:59Z
dc.date.issued2002-05-31
dc.identifier.citationTelomeric localization of the vertebrate-type hexamer repeat, (TTAGGG) ₙ, in the wedgeshell clam "Donax trunculus" and other marine invertebrate genomes. Miroslav Plohl, Eva Prats, Andrés Martínez-Lage, Ana González-Tizón, Josefina Méndez and Luis Cornudella, J Biol. Chem., 277(22), 2002, 19839–19846.es_ES
dc.identifier.issn1083-351X
dc.identifier.urihttp://hdl.handle.net/2183/25807
dc.description.abstract[Abstract]: The hexamer repeat sequence (TTAGGG) n, found at the ends of all vertebrate chromosomes, was previously identified as the main building element of one member of aHindIII satellite DNA family characterized in the genome of the bivalve mollusc "Donax trunculus". It was also found in 22 perfect tandem repeats in a cloned junction region juxtaposed to the proper satellite sequence, from which the DNA tract encompassing the clustered tandem copies was excised and subcloned. Here, the chromosomal distribution of (TTAGGG) n sequences in the "Donax" was studied by the sensitivity to Bal31 exonuclease digestion, fluorescence in situ hybridization (FISH) on metaphase chromosomes and rotating-field gel electrophoresis. To verify the occurrence of the hexamer repeat in the genomes of taxonomically related molluscs and other marine invertebrates, genomic DNA from the mussel "Mytilus galloprovincialis" and the echinoderm "Holothuria tubulosa" was also analyzed. The kinetics of Bal31 hydrolysis of high molecular mass DNA from the three marine invertebrates revealed a marked decrease over time of the hybridization with the cloned (TTAGGG)22 sequence, concomitantly with a progressive shortening of the positively reacting DNA fragments. This revealed a marked susceptibility to exonuclease consistent with terminal positioning on the respective chromosomal DNAs. In full agreement, FISH results with the (TTAGGG) 22 probe showed that the repeat appears located in telomeric regions in all chromosomes of both bivalve molluscs. The presence of (TTAGGG) n repeat tracts in marine invertebrate telomeres points to its wider distribution among eukaryotic organisms and suggests an ancestry older than originally presumed from its vertebrate distinctiveness.es_ES
dc.description.sponsorshipEspaña. Dirección General de Enseñanza Superior; PB97-1136es_ES
dc.description.sponsorshipCataluña. Direcció General de Recerca; SGR01-354es_ES
dc.language.isoenges_ES
dc.publisherAmerican Society for Biochemistry and Molecular Biologyes_ES
dc.relation.urihttps://doi.org/10.1074/jbc.M201032200es_ES
dc.rightsThis is a post-peer-review, pre-copyedit version of an article published in [Journal of Biological Chemistry]. The final authenticated version is available online at: [doi: 10.1074/jbc.M201032200].es_ES
dc.titleTelomeric localization of the vertebrate-type hexamer repeat, (TTAGGG) ₙ, in the wedgeshell clam "Donax trunculus" and other marine invertebrate genomeses_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.rights.accessinfo:eu-repo/semantics/openAccesses_ES
UDC.journalTitleJournal of Biological Chemistryes_ES
UDC.volume277es_ES
UDC.issue22es_ES
UDC.startPage19839es_ES
UDC.endPage19846es_ES


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