Isolation and characterization of polymorphic microsatellite loci in the razor clam "Ensis siliqua"

Varela, Miguel A.; González-Tizón, Ana M.; Francisco Candeira, Marta; Martínez-Lage, Andrés

Title

Author(s)

Varela, Miguel A.

González-Tizón, Ana M.

Francisco Candeira, Marta

Martínez-Lage, Andrés

Date

2007-03

Citation

Varela, M. A., González-Tizón, A., Francisco-Candeira, M. and Martínez-Lage, A. (2007), Isolation and characterization of polymorphic microsatellite loci in the razor clam Ensis siliqua. Molecular Ecology Notes, 7(2): 221-222.

Abstract

[Abstract] Five polymorphic microsatellite loci in the razor clam Ensis siliqua are described. A collection consisting of 34 individuals from Finisterre, Spain, was analysed. Loci were isolated from the sequences of intersimple sequence repeat (ISSR) markers. Detailed analysis of 42 ISSR markers led to the design of 16 primer pairs. Five of these yielded consistent and polymorphic products. The number of alleles ranged from five to 23 per locus with the observed heterozygosity ranging from 0.46 to 0.94. Linkage equilibrium was observed in all loci and three of them showed significant deviations from Hardy–Weinberg equilibrium.

Keywords

Ensis siliqua
ISSR
Microsatellites
Razor clam

Editor version

https://doi.org/10.1111/j.1471-8286.2006.01423.x

Rights

This is the peer reviewed version of the following article: [Varela, M. A., González-Tizón, A., Francisco-Candeira, M. and Martínez-Lage, A. (2007), Isolation and characterization of polymorphic microsatellite loci in the razor clam Ensis siliqua. Molecular Ecology Notes, 7(2): 221-222.], which has been published in final form at [https://doi.org/10.1111/j.1471-8286.2006.01423.x]. This article may be used for non-commercial purposes in accordance with Wiley terms and conditions for use of self-archived versions.