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Genetic divergence detected by ISSR markers and characterization of microsatellite regions in "Mytilus" mussels

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Martinez_Lage_Andres_2007_Genetic_divergence_detected_ISSR_markers.pdf (643.5Kb)
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http://hdl.handle.net/2183/21429
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  • Investigación (FCIE) [1260]
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Título
Genetic divergence detected by ISSR markers and characterization of microsatellite regions in "Mytilus" mussels
Autor(es)
Varela, Miguel A.
González-Tizón, Ana M.
Mariñas-Pardo, Luis
Martínez-Lage, Andrés
Data
2007-08
Cita bibliográfica
Genetic divergence detected by ISSR markers and characterization of microsatellite regions in Mytilus mussels. Miguel A. Varela, Ana González-Tizón, Luis Mariñas, Andrés Martínez-Lage, Biochem. Genet. (2007), 45(7-8): 565-578.
Resumo
[Abstract] The wide distribution of microsatellites in mussels of the Mytilus edulis complex (Mytilidae) enables the analysis of inter-simple-sequence repeat (ISSR) markers. The aim of this investigation was to assess genetic differentiation in six sampling localities distributed along the European Atlantic coast to expose the potential of these markers in genetic studies requiring the detection of low polymorphism and as a source of sequences for developing microsatellite markers. We detected low genetic structuring within each member of the Mytilus edulis complex. Nei and Li distances and AMOVA clustered the individuals studied into two groups. On the basis of these results two sampling localities coming from the M. edulis × M. galloprovincialis mosaic hybrid zone in Western Europe were assigned to one species. On the other hand, mussels of a sampling locality in the Baltic Sea were not significantly different from a pure M. edulis locality supporting an extensive introgression of M. edulis in these individuals. Finally, 148 microsatellites were found in the sequences of 51 ISSR markers, and two polymorphic microsatellite markers were developed.
Palabras chave
Genetic variation
ISSR
Microsatellites
Mussel
Mytilus
 
Versión do editor
https://doi.org/10.1007/s10528-007-9097-7
Dereitos
This is a post-peer-review, pre-copyedit version of an article published in [Biochemical Genetics]. The final authenticated version is available online at: [https://doi.org/10.1007/s10528-007-9097-7].
ISSN
1573-4927
0006-2928
 

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