RNA-Seq analysis for assessing the early response to DSP toxins in Mytilus galloprovincialis digestive gland and gill
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RNA-Seq analysis for assessing the early response to DSP toxins in Mytilus galloprovincialis digestive gland and gillData
2018-10-16Cita bibliográfica
Prego-Faraldo, M.V.; Martínez, L.; Méndez, J. RNA-Seq Analysis for Assessing the Early Response to DSP Toxins in Mytilus galloprovincialis Digestive Gland and Gill. Toxins 2018, 10, 417.
Resumo
[Abstract] The harmful effects of diarrhetic shellfish poisoning (DSP) toxins on mammalian cell lines have been widely assessed. Studies in bivalves suggest that mussels display a resistance to the cytogenotoxic effects of DSP toxins. Further, it seems that the bigger the exposure, the more resistant mussels become. To elucidate the early genetic response of mussels against these toxins, the digestive gland and the gill transcriptomes of Mytilus galloprovincialis after Prorocentrum lima exposure (100,000 cells/L, 48 h) were de novo assembled based on the sequencing of 8 cDNA libraries obtained using an Illumina HiSeq 2000 platform. The assembly provided 95,702 contigs. A total of 2286 and 4523 differentially expressed transcripts were obtained in the digestive gland and the gill, respectively, indicating tissue-specific transcriptome responses. These transcripts were annotated and functionally enriched, showing 44 and 60 significant Pfam families in the digestive gland and the gill, respectively. Quantitative PCR (qPCR) was performed to validate the differential expression patterns of several genes related to lipid and carbohydrate metabolism, energy production, genome integrity and defense, suggesting their participation in the protective mechanism. This work provides knowledge of the early response against DSP toxins in the mussel M. galloprovincialis and useful information for further research on the molecular mechanisms of the bivalve resistance to these toxins.
Palabras chave
DSP toxins
Bivalves
Mussel
Resistance
RNA-Seq
qPCR
Metabolism
Defense
Immunity
Bivalves
Mussel
Resistance
RNA-Seq
qPCR
Metabolism
Defense
Immunity
Versión do editor
Dereitos
Atribución 3.0 España
ISSN
2072-665