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Early alterations on photosynthesis-related parameters in Chlamydomonas reinhardtii cells exposed to atrazine: A multiple approach study
dc.contributor.author | Esperanza, Marta | |
dc.contributor.author | Seoane, Marta | |
dc.contributor.author | Rioboo, Carmen | |
dc.contributor.author | Herrero, Concepción | |
dc.contributor.author | Cid, Ángeles | |
dc.date.accessioned | 2016-03-09T16:09:11Z | |
dc.date.issued | 2016-06-01 | |
dc.identifier.citation | Esperanza M, Seoane M, Rioboo C, Herrero C, Cid Á. Early alterations on photosynthesis-related parameters in Chlamydomonas reinhardtii cells exposed to atrazine: A multiple approach study. Science of The Total Environment, 2016; 554-555: 237–45. doi:10.1016/j.scitotenv.2016.02.175 | es_ES |
dc.identifier.issn | 0048-9697 | |
dc.identifier.uri | http://hdl.handle.net/2183/16287 | |
dc.description.abstract | Chlamydomonas reinhardtii cells were exposed to a sublethal concentration of thewidespread herbicide atrazine for 3 h. Physiological cellular parameters, such as chlorophyll a fluorescence and oxidative stress monitored by flow cytometry and pigments levels were altered in microalgal cells exposed to 0.25 μMof atrazine. Furthermore, the effects of this herbicide on C. reinhardtii were explored using “omics” techniques. Transcriptomic analyses, carried out by RNA-Seq technique, displayed 9 differentially expressed genes, related to photosynthesis, between control cultures and atrazine exposed cultures. Proteomic profiles were obtained using iTRAQ tags and MALDI-MS/MS analysis, identifying important changes in the proteome during atrazine stress; 5 proteins related to photosynthesis were downexpressed. The results of these experiments advance the understanding of photosynthetic adjustments that occur during an early herbicide exposure. Inhibition of photosynthesis induced by atrazine toxicity will affect the entire physiological and biochemical states of microalgal cells. | es_ES |
dc.description.sponsorship | Ministerio de Economía y Competitividad; CGL2010-15993/BOS | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Elsevier | es_ES |
dc.relation.uri | http://dx.doi.org/10.1016/j.scitotenv.2016.02.175 | es_ES |
dc.subject | iTRAQ | es_ES |
dc.subject | RNA-Seq | es_ES |
dc.subject | Microalga | es_ES |
dc.subject | Atrazine | es_ES |
dc.subject | Flow cytometry | es_ES |
dc.subject | MALDI | es_ES |
dc.subject | Chlamydomonas reinhardtii | es_ES |
dc.title | Early alterations on photosynthesis-related parameters in Chlamydomonas reinhardtii cells exposed to atrazine: A multiple approach study | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.access | info:eu-repo/semantics/openAccess | es_ES |
dc.date.embargoEndDate | 2018-06-01 | es_ES |
dc.date.embargoLift | 2018-06-01 |