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dc.contributor.authorFábregas, Jaime
dc.contributor.authorHerrero, Concepción
dc.contributor.authorCabezas, Buenaventura
dc.contributor.authorAbalde, Julio
dc.date.accessioned2015-09-09T15:04:07Z
dc.date.available2015-09-09T15:04:07Z
dc.date.issued1985
dc.identifier.citationFabregas J, Herrero C, Cabezas B, Abalde J. Mass culture and biochemical variability of the marine microalga Tetraselmis suecica Kylin (Butch) with high nutrient concentrations. Aquaculture 1985;49(3-4):231-244. http://dx.doi.org/10.1016/0044-8486(85)90082-1es_ES
dc.identifier.issn044-8486
dc.identifier.urihttp://hdl.handle.net/2183/14911
dc.description.abstractMass cultures of Tetraselmis suecica were carried out with four nutrient concentrations, ranging from 2 to 16 mM of NaNO3 and salinity 35‰. An air flow of 15 l/min maintained a CO2 transference rate sufficient to keep the pH below 8.4. Using these cultural conditions equations were calculated, by a multiple non-linear least squares regression of order four, enabling predictions to be made of growth kinetics and chemical composition. Maximum cellular densities of 7.83 × 106 and 7.15 × 106 cells/ml were obtained with 8 and 16 mM of NaNO3, respectively. Growth velocity ranged between 0.53 and 0.63 doublings (dbl)/day, although 0.98 dbl/day were reached with 16 mM of NaNO3. Volume increased with nutrient concentration from 252 to 905 μm3. Protein content reached maximum values of 306 μg/ml or 59.8 pg/cell. In the logarithmic phase, protein was regulated by nutrient concentration and decreased according to this concentration. Maximum efficiency of transformation from nitrate to protein was 108%, obtained at 2 mM of NaNO3. Efficiency decreased, to 14%, when nutrient concentration increased. This fact indicates that the lowest cost of harvesting is obtained with a nutrient concentration of 2 mM NaNO3. Chlorophyll a cell reached values between 3.1 and 3.8 pg/cell in the stationary phase. There was a relationship between nutrient concentration and chlorophyll α cell in the logarithmic phase, with an increase from 2.15 pg/cell to 3.74 pg/cell. Changes in chlorophyll α level are related to nitrogen depletion. Carbohydrate/cell was constant at values of 19.84-28.68 pg/cell in the logarithmic and stationary phases and was not related to nitrogen depletion. RNA/cell ranged from 4.17 to 5.48 pg/cell, except at 2 mM of NaNO3 when it was 2.77 pg/cell, probably due to nitrogen depletion. The level of DNA/cell was constant in all the nutrient concentrations assayed and ranged from 0.1 to 1.09 pg/cell. Great variability in the chemical composition of T. suecica has been shown. Growth in mass cultures is closely coupled to changes in nutrient concentrations and variations occur in protein, chlorophyll α and RNA content, showing differences of 215%, 190% and 203%, respectively, in the stationary phase. This biochemical variability, mainly in protein content, must have a marked effect on the nutritive value of this microalga as feed in mariculture.es_ES
dc.language.isoenges_ES
dc.publisherElsevier Science Publisherses_ES
dc.relation.urihttp://dx.doi.org/10.1016/0044-8486(85)90082-1es_ES
dc.subjectTetraselmis suecicaes_ES
dc.subjectNutrient concentrationses_ES
dc.subjectCultureses_ES
dc.subjectBiochemical variabilityes_ES
dc.subjectMarine microalgaees_ES
dc.titleMass culture and biochemical variability of the marine microalga Tetraselmis suecica Kylin (Butch) with high nutrient concentrationses_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.rights.accessinfo:eu-repo/semantics/openAccesses_ES


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