Listar por autor "Rey-Rico, Ana"
Mostrando ítems 1-20 de 25
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Biomaterial-Assisted Gene Therapy for Translational Approaches to Treat Musculoskeletal Disorders
Venkatesan, Jagadeesh Kumar; Rey-Rico, Ana; Meng, Weikun; Cai, Xiaoyu; Pons, Françoise; Lebeau, Luc; Migonney, Véronique; Madry, Henning; Cucchiarini, Magali (Elsevier, 2020-12-28)[Abstract] Biomaterial-assisted gene therapy is a promising strategy for the treatment of various musculoskeletal disorders such as those concerning the articular cartilage, bones, tendons and ligaments, and meniscus as ... -
Chondrogenic Differentiation of Human Mesenchymal Stem Cells via SOX9 Delivery in Cationic Niosomes
Carballo-Pedrares, Natalia; Sanjurjo-Rodríguez, Clara; Señarís, José; Díaz-Prado, Silvia; Rey-Rico, Ana (MDPI, 2022-10-28)[Abstract] Gene transfer to mesenchymal stem cells constitutes a powerful approach to promote their differentiation into the appropriate cartilage phenotype. Although viral vectors represent gold standard vehicles, because ... -
Controlled Release of rAAV Vectors from APMA-Functionalized Contact Lenses for Corneal Gene Therapy
Álvarez-Rivera, Fernando; Rey-Rico, Ana; Venkatesan, Jagadeesh K.; Díaz-Gómez, Luis; Cucchiarini, Magali; Concheiro, Ángel; Álvarez-Lorenzo, Carmen (MDPI, 2020-04-09)[Abstract] As an alternative to eye drops and ocular injections for gene therapy, the aim of this work was to design for the first time hydrogel contact lenses that can act as platforms for the controlled delivery of ... -
Current trends in viral gene therapy for human orthopaedic regenerative medicine
Venkatesan, Jagadeesh Kumar; Rey-Rico, Ana; Cucchiarini, Magali (Springer, 2019-02-21)[Abstract] Background: Viral vector-based therapeutic gene therapy is a potent strategy to enhance the intrinsic reparative abilities of human orthopaedic tissues. However, clinical application of viral gene transfer remains ... -
Development of New Non-viral Systems for Genetic Modification of Senescent Cells
López-Seijas, Junquera; Miranda-Balbuena, Diego; Iglesias-Fente, Alba; Sacristán-Santos, Marta; Carballo-Pedrares, Natalia; Arufe, M.C.; Rey-Rico, Ana; Fafián Labora, Juan Antonio (Cell Press, 2023-03-20)[Abstract] Senescence is a process characterized by a prolonged irreversible cell-cycle arrest. The accumulation of senescent cells in tissues is related to aging and to the development of age-related diseases. Recently, ... -
Effective remodelling of human osteoarthritic cartilage by sox9 gene transfer and overexpression upon delivery of rAAV vectors in polymeric micelles
Rey-Rico, Ana; Venkatesan, Jagadesh K.; Schmitt, Gertrud; Speicher-Mentges, Susanne; Madry, Henning; Cucchiarini, Magali (American Chemical Society, 2018-05-15)[Abstract] Recombinant adeno-associated virus (rAAV) vectors are well suited carriers to provide durable treatments for human osteoarthritis (OA). Controlled release of rAAV from polymeric micelles was already shown to ... -
Effects of rAAV-Mediated sox9 Overexpression on the Biological Activities of Human Osteoarthritic Articular Chondrocytes in Their Intrinsic Three-Dimensional Environment
Daniels, Oliver; Rey-Rico, Ana; Schmitt, Gertrud; Venkatesan, Jagadeesh Kumar; Cucchiarini, Magali; Frisch, Janina (MDPI, 2019)[Abstract] Gene therapy for osteoarthritis offers powerful, long-lasting tools that are well adapted to treat such a slow, progressive disorder, especially those therapies based on the clinically adapted recombinant ... -
Enhanced Chondrogenic Differentiation Activities in Human Bone Marrow Aspirates via sox9 Overexpression Mediated by pNaSS-Grafted PCL Film-Guided rAAV Gene Transfer
Venkatesan, Jagadeesh Kumar; Rey-Rico, Ana; Meng, Weikun; Schmitt, Gertrud; Speicher-Mentges, Susanne; Falentin-Daudré, Céline; Leroux, Amélie; Madry, Henning; Migonney, Véronique; Cucchiarini, Magali (MDPI, 2020)[Abstract] Background: The delivery of therapeutic genes in sites of articular cartilage lesions using non-invasive, scaffold-guided gene therapy procedures is a promising approach to stimulate cartilage repair while ... -
Gene-Activated Hyaluronic Acid-Based Cryogels for Cartilage Tissue Engineering
López-Seijas, Junquera; Rey-Rico, Ana; Carballo-Pedrares, Natalia; Miranda-Balbuena, Diego; Lamas Criado, Ibán; Yáñez, Julián (Elsevier, 2023)[Abstract] Articular cartilage injuries are very frequent lesions that if left untreated may degenerate into osteoarthritis. Gene transfer to mesenchymal stem cells (MSCs) provides a powerful approach to treat these lesions ... -
Hydrogel-based localized nonviral gene delivery in regenerative medicine approaches: an overview
Carballo-Pedrares, Natalia; Fuentes Boquete, Isaac Manuel; Díaz-Prado, Silvia; Rey-Rico, Ana (MDPI, 2020-08-10)[Abstract] Hydrogel-based nonviral gene delivery constitutes a powerful strategy in various regenerative medicine scenarios, as those concerning the treatment of musculoskeletal, cardiovascular, or neural tissues disorders ... -
Hydrogel-Guided, rAAV-Mediated IGF-I Overexpression Enables Long-Term Cartilage Repair and Protection against Perifocal Osteoarthritis in a Large-Animal Full-Thickness Chondral Defect Model at One Year In Vivo
Rey-Rico, Ana; Madry, Henning; Venkatesan, Jagadeesh Kumar; Schmitt, Gertrud; Speicher-Mentges, Susanne; Cai, Xiaoyu; Meng, Weikun; Cucchiarini, Magali; Maihöfer, Johanna; Goebel, Lars; Zurakowski, David; Menger, Michael D.; Laschke, Matthias W. (Wiley-VCH, 2021)[Abstract] The regeneration of focal articular cartilage defects is complicated by the reduced quality of the repair tissue and the potential development of perifocal osteoarthritis (OA). Biomaterial-guided gene therapy ... -
Improved Chondrogenic Differentiation of rAAV SOX9-Modified Human MSCs Seeded in Fibrin-Polyurethane Scaffolds in a Hydrodynamic Environment
Venkatesan, Jagadeesh Kumar; Rey-Rico, Ana; Cucchiarini, Magali; Madry, Henning; Gardner, Oliver; Eglin, David; Alini, Mauro; Stoddart, Martin J. (MDPI, 2018)[Abstract] The repair of focal articular cartilage defects remains a problem. Combining gene therapy with tissue engineering approaches using bone marrow-derived mesenchymal stem cells (MSCs) may allow the development of ... -
Niosomes-Based Gene Delivery Systems for Effective Transfection of Human Mesenchymal Stem Cells
Carballo-Pedrares, Natalia; Kattar, Axel; Concheiro, Ángel; Álvarez-Lorenzo, Carmen; Rey-Rico, Ana (Elsevier, 2021-07-11)[Abstract] Gene transfer to mesenchymal stem cells (MSCs) has arisen as a powerful approach to increase the therapeutic potential of this effective cell population. Over recent years, niosomes have emerged as self-assembled ... -
Non-viral Gene Delivery to Human Mesenchymal Stem Cells: A Practical Guide Towards Cell Engineering
López-Seijas, Junquera; Rey-Rico, Ana; Carballo-Pedrares, Natalia; Ponti, Federica; Miranda-Balbuena, Diego; Bono, Nina; Candiani, Gabriele (BioMed Central, 2023)[Abstract] In recent decades, human mesenchymal stem cells (hMSCs) have gained momentum in the field of cell therapy for treating cartilage and bone injuries. Despite the tri-lineage multipotency, proliferative properties, ... -
PEO-PPO-PEO Tri-Block Copolymers for Gene Delivery Applications in Human Regenerative Medicine: an overview
Rey-Rico, Ana; Cucchiarini, Magali (2018)[Abstract:] Lineal (poloxamers or Pluronic®) or X-shaped (poloxamines or Tetronic®) amphiphilic tri-block copolymers of poly(ethylene oxide) and poly(propylene oxide) (PEO-PPO-PEO) have been broadly explored for controlled ... -
PEO-PPO-PEO tri-block copolymers for gene delivery applications in human regenerative medicine: an overview
Rey-Rico, Ana; Cucchiarini, Magali (MDPI, 2018-03-08)[Abstract] Lineal (poloxamers or Pluronic®) or X-shaped (poloxamines or Tetronic®) amphiphilic tri-block copolymers of poly(ethylene oxide) and poly(propylene oxide) (PEO-PPO-PEO) have been broadly explored for controlled ... -
rAAV-Mediated Overexpression of SOX9 and TGF-β via Carbon Dot-Guided Vector Delivery Enhances the Biological Activities in Human Bone Marrow-Derived Mesenchymal Stromal Cells
Meng, Weikun; Rey-Rico, Ana; Claudel, Mickaël; Schmitt, Gertrud; Speicher-Mentges, Susanne; Pons, Françoise; Lebeau, Luc; Venkatesan, Jagadeesh K.; Cucchiarini, Magali (MDPI, 2020-04-28)[Abstract] Scaffold-assisted gene therapy is a highly promising tool to treat articular cartilage lesions upon direct delivery of chondrogenic candidate sequences. The goal of this study was to examine the feasibility ... -
Remodeling of human osteochondral defects via rAAV-mediated co-overexpression of TGF-β and IGF-I from implanted human bone marrow-derived mesenchymal stromal cells
Morscheid, Stephanie; Venkatesan, Jagadeesh Kumar; Rey-Rico, Ana; Schmitt, Gertrud; Cucchiarini, Magali (MDPI, 2019-08-28)[Abstract] The application of chondrogenic gene sequences to human bone marrow-derived mesenchymal stromal cells (hMSCs) is an attractive strategy to activate the reparative activities of these cells as a means to enhance ... -
Scaffold-Mediated Gene Delivery for Osteochondral Repair
Madry, Henning; Venkatesan, Jagadeesh Kumar; Carballo-Pedrares, Natalia; Rey-Rico, Ana; Cucchiarini, Magali (MDPI, 2020-09-29)[Abstract] Osteochondral defects involve both the articular cartilage and the underlying subchondral bone. If left untreated, they may lead to osteoarthritis. Advanced biomaterial-guided delivery of gene vectors has ... -
Supramolecular Cyclodextrin-Based Hydrogels for Controlled Gene Delivery
Rey-Rico, Ana; Cucchiarini, Magali (2019)[Abstract] Controlled delivery of gene transfer vectors is a powerful strategy to enhance the temporal and spatial presentation of therapeutic agents in a defined target. Hydrogels are adapted biomaterials for gene delivery ...
