Listar por autor "Balado, Miguel"
Mostrando ítems 1-14 de 14
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Detection of Aeromonas Salmonicida Subsp. Salmonicida Infection in Zebrafish by Labelling Bacteria With Gfp and a Fluorescent Probe Based on the Siderophore Amonabactin
Rodríguez-Pedrouzo, A.; Cisneros Sureda, Javier; Martínez Matamoros, Diana; Rey-Varela, Diego; Balado, Miguel; Rodríguez, Jaime; Lemos, Manuel L.; Folgueira, Mónica; Jiménez, Carlos (Elsevier, 2023-10-21)[Abstract] Zebrafish (Danio rerio) is an excellent model to study bacterial infections in fish and their treatment. We used zebrafish as a model of infection for Aeromonas salmonicida subsp. salmonicida (hereinafter A. ... -
FrpA Is the Outer Membrane Piscibactin Transporter in Vibrio Anguillarum: Structural Elements in Synthetic Piscibactin Analogues Required for Transport
Lages, Marta Afonso; De la Fuente, Carmen; Ageitos, Lucía; Martínez Matamoros, Diana; Rodríguez, Jaime; Balado, Miguel; Jiménez, Carlos; Lemos, Manuel L. (Springer Nature, 2021-11-18)[Abstract] Piscibactin (Pcb) is a labile siderophore widespread among Vibrionaceae. Its production is a major virulence factor of some fish pathogens such as Photobacterium damselae subsp. piscicida and Vibrio anguillarum. ... -
Identification of Key Functions Required for Production and Utilization of the Siderophore Piscibactin Encoded by the High-Pathogenicity Island irp-HPI in Vibrionaceae
Lages, Marta Afonso; Ageitos, Lucía; Rodríguez, Jaime; Jiménez, Carlos; Lemos, Manuel L.; Balado, Miguel (MDPI, 2022)[Abstract] Piscibactin is a widespread siderophore system present in many different bacteria, especially within the Vibrionaceae family. Previous works showed that most functions required for biosynthesis and transport of ... -
O-versus S-Metal Coordination of the Thiocarboxylate Group: An NMR Study of the Two Tautomeric Forms of the Ga(III)-Photoxenobactin E Complex
Buedenbender, Larissa; Ageitos, Lucía; Lages, Marta Afonso; Platas-Iglesias, Carlos; Balado, Miguel; Lemos, Manuel L.; Rodríguez, Jaime; Jiménez, Carlos (American Chemical Society, 2024-02-22)[Abstract] Photoxenobactin E (1) is a natural product with an unusual thiocarboxylic acid terminus recently isolated from an entomopathogenic bacterium. The biosynthetic gene cluster associated with photoxenobactin E, and ... -
Outer Membrane Protein FrpA, the Siderophore Piscibactin Receptor of Photobacterium damselae subsp. piscicida, as a Subunit Vaccine Against Photobacteriosis in Sole (Solea senegalensis)
Valderrama Pereira, Andrea Katherine; Balado, Miguel; Rey-Varela, Diego; Rodríguez, Jaime; Vila-Sanjurjo, Antón; Jiménez, Carlos; Lemos, Manuel L. (Elsevier, 2019-09-30)[Abstract] Photobacteriosis caused by Photobacterium damselae subsp. piscicida (Pdp) remains one of the main infectious diseases affecting cultured fish in Mediterranean countries. Diverse vaccine formulations based in the ... -
Preparation of Functionalized Magnetic Nanoparticles Conjugated with Feroxamine and Their Evaluation for Pathogen Detection
Martínez Matamoros, Diana; Castro-García, Socorro; Balado, Miguel; Matamoros Veloza, Adriana; Camargo-Valero, Miller Alonso; Óscar, Céspedes; Rodríguez, Jaime; Lemos, Manuel L.; Jiménez, Carlos (Royal Society of Chemistry, 2019-05-01)[Abstract] This work reports the preparation of a conjugate between amino-functionalized silica magnetite and the siderophore feroxamine. The morphology and properties of the conjugate and intermediate magnetic nanoparticles ... -
Selective Detection of Aeromonas Spp. By a Fluorescent Probe Based on the Siderophore Amonabactin
Cisneros Sureda, Javier; Rey-Varela, Diego; Rodríguez, Jaime; Balado, Miguel; Lemos, Manuel L.; Jiménez, Carlos (Elsevier, 2022-02-03)[Abstract] Amonabactins, the siderophores produced by some pathogenic bacteria belonging to Aeromonas genus, can be used for the preparation of conjugates to be imported into the cell using their specific transport machinery. ... -
Structural Requirements for Ga3+ Coordination in Synthetic Analogues of the Siderophore Piscibactin Deduced by Chemical Synthesis and Density Functional Theory Calculations
De la Fuente, Carmen; Ageitos, Lucía; Lages, Marta Afonso; Martínez Matamoros, Diana; Forero, Abel M.; Balado, Miguel; Lemos, Manuel L.; Rodríguez, Jaime; Jiménez, Carlos (American Chemical Society, 2023-05-04)[Abstract] Stereoselective total synthesis of several analogues of piscibactin (Pcb), the siderophore produced by different pathogenic Gram-negative bacteria, was performed. The acid-sensitive α-methylthiazoline moiety was ... -
Synthesis of Functionalized Magnetic Nanoparticles, Their Conjugation with the Siderophore Feroxamine and its Evaluation for Bacteria Detection
Martínez Matamoros, Diana; Castro-García, Socorro; Ojeda-Romano, Gabriela; Balado, Miguel; Rodríguez, Jaime; Lemos, Manuel L.; Jiménez, Carlos (JoVE, 2020-06-16)[Abstract] In the present work, the synthesis of magnetic nanoparticles, its coating with SiO2, followed by its amine functionalization with (3-aminopropyl)triethoxysilane (APTES) and its conjugation with deferoxamine, a ... -
The Fish Pathogen Vibrio ordalii Under Iron Deprivation Produces the Siderophore Piscibactin
Ruiz, Pamela; Balado, Miguel; Fuentes-Monteverde, Juan Carlos; Toranzo, Alicia E.; Rodríguez, Jaime; Jiménez, Carlos; Avendaño-Herrera, Rubén; Lemos, Manuel L. (MDPI, 2019-09-03)[Abstract] Vibrio ordalii is the causative agent of vibriosis, mainly in salmonid fishes, and its virulence mechanisms are still not completely understood. In previous works we demonstrated that V. ordalii possess several ... -
The Marine Bivalve Molluscs Pathogen Vibrio neptunius Produces the Siderophore Amphibactin, Which Is Widespread in Molluscs Microbiota
Galvis, Fabián; Ageitos, Lucía; Martínez Matamoros, Diana; Barja, Juan L.; Rodríguez, Jaime; Lemos, Manuel L.; Jiménez, Carlos; Balado, Miguel (Wiley, 2020-12-01)[Abstract] Amphiphilic siderophores, including amphibactins, are the most abundant siderophores in oceans. Genes putatively encoding the amphibactin system were proposed in some bacteria and homologues of these genes are ... -
The Outer Membrane Protein FstC of Aeromonas salmonicida subsp. salmonicida Acts as Receptor for Amonabactin Siderophores and Displays a Wide Ligand Plasticity. Structure–Activity Relationships of Synthetic Amonabactin Analogues
Rey-Varela, Diego; Cisneros Sureda, Javier; Balado, Miguel; Rodríguez, Jaime; Lemos, Manuel L.; Jiménez, Carlos (American Chemical Society, 2019-09-26)[Abstract] Amonabactins are a group of four related catecholate siderophores produced by several species of the genus Aeromonas, including A. hydrophila and the fish pathogen A. salmonicida subsp. salmonicida. Although the ... -
The Siderophore Piscibactin Is a Relevant Virulence Factor for Vibrio anguillarum Favored at Low Temperatures
Balado, Miguel; Lages, Marta Afonso; Fuentes-Monteverde, Juan Carlos; Martínez Matamoros, Diana; Rodríguez, Jaime; Jiménez, Carlos; Lemos, Manuel L. (Frontiers, 2018-08-02)[Abstract] Vibrio anguillarum causes vibriosis, a hemorrhagic septicaemia that affects many cultured marine fish species worldwide. Two catechol siderophores, vanchrobactin and anguibactin, were previously identified in ... -
Vibrio neptunius Produces Piscibactin and Amphibactin and Both Siderophores Contribute Significantly to Virulence for Clams
Galvis, Fabián; Ageitos, Lucía; Rodríguez, Jaime; Jiménez, Carlos; Barja, Juan L.; Lemos, Manuel L.; Balado, Miguel (Frontiers, 2021-10-25)[Abstract] Vibrio neptunius is an inhabitant of mollusc microbiota and an opportunistic pathogen causing disease outbreaks in marine bivalve mollusc species including oysters and clams. Virulence of mollusc pathogenic ...
