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Development of new non-viral systems for genetic modification of senescent cells
dc.contributor.author | López-Seijas, Junquera | |
dc.contributor.author | Miranda-Balbuena, Diego | |
dc.contributor.author | Iglesias-Fente, Alba | |
dc.contributor.author | Sacristán-Santos, Marta | |
dc.contributor.author | Carballo-Pedrares, Natalia | |
dc.contributor.author | Arufe, M.C. | |
dc.contributor.author | Rey-Rico, Ana | |
dc.contributor.author | Fafián Labora, Juan Antonio | |
dc.date.accessioned | 2023-04-17T07:06:22Z | |
dc.date.available | 2023-04-17T07:06:22Z | |
dc.date.issued | 2023-03-20 | |
dc.identifier.citation | López-Seijas J, Miranda-Balbuena D, Iglesias-Fente A, Sacristán-Santos M, Carballo-Pedrares N, Arufe MC, et al. Development of new non-viral systems for genetic modification of senescent cells. Mol Ther Nucleid Acids. 2023 Jun;32:302-217. | es_ES |
dc.identifier.issn | 2329-0501 | |
dc.identifier.uri | http://hdl.handle.net/2183/32875 | |
dc.description.abstract | [Abstract] Senescence is a process characterized by a prolonged irreversible cell-cycle arrest. The accumulation of senescent cells in tissues is related to aging and to the development of age-related diseases. Recently, gene therapy has emerged as a powerful tool for treating age-associated diseases by the transference of specific genes into the target cell population. However, the high sensitivity of senescent cells significantly precludes their genetic modification via classical viral and non-viral systems. Niosomes are self-assembled non-viral nanocarriers that exhibit important advantages due to their elevated cytocompatibility, versatility, and cost-efficiency, arising as a new alternative for genetic modification of senescent cells. In this work, we explore for the first time the use of niosomes for genetic modification of senescent umbilical cord-derived mesenchymal stem cells. We report that niosome composition greatly affected transfection efficiency; those formulations prepared in medium with sucrose and containing cholesterol as helper lipid being the most suitable to transfect senescent cells. Moreover, resulting niosome formulations exhibited a superior transfection efficiency with a markedly less cytotoxicity than the commercial reagent Lipofectamine. These findings highlight the potentiality of niosomes as effective vectors for genetic modification of senescent cells, providing new tools for the prevention and/or treatment of age-related diseases. | es_ES |
dc.description.sponsorship | The work was supported by MICINN (RTI2018-099389-A-100; RYC2018-025617-I; RYC2021-032567-I), Xunta de Galicia (ED431F2021/10; ED481D-2021-020), and Spanish National Health Institute Carlos III (PI20/00497). J.L.-S. acknowledges MICINN (FPU20/06176) for her pre-doctoral fellowship grant. J.F.-L. also gives thanks to the InTalent program from UDC-Inditex for the research grant. | es_ES |
dc.description.sponsorship | Ministerio de Ciencia, Innovación y Universidades (España); RTI2018-099389-A-100 | es_ES |
dc.description.sponsorship | Ministerio de Ciencia, Innovación y Universidades (España); RYC2018-025617-I | es_ES |
dc.description.sponsorship | Xunta de Galicia; ED431F2021/10 | es_ES |
dc.description.sponsorship | Xunta de Galicia; ED481D-2021-020 | es_ES |
dc.description.sponsorship | info:eu-repo/grantAgreement/ISCIII/Programa Estatal de Generación de Conocimiento y Fortalecimiento del Sistema Español de I+D+I/PI20%2F00497/ES/TERAPIA CELULAR CON MICRO ARN Y VESICULAS EXTRACELULARES PARA EL TRATAMIENTO DE LA INFLAMACION CRONICA EN UN MODELO DE OA. (TERAPIA LIBRE DE CELULAS) | es_ES |
dc.description.sponsorship | Ministerio de Ciencia, Innovación y Universidades (España); RYC2021-032567-I | |
dc.language.iso | eng | es_ES |
dc.publisher | Cell Press | es_ES |
dc.relation.uri | https://doi.org/10.1016/j.omtn.2023.03.010 | es_ES |
dc.rights | Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC-BY-NC-ND 4.0) | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Delivery strategies | es_ES |
dc.subject | Senescence | es_ES |
dc.subject | Aging | es_ES |
dc.subject | Umbilical cord mesenchymal stem cells | es_ES |
dc.subject | Gene therapy | es_ES |
dc.subject | Non-viral vectors | es_ES |
dc.subject | Niosomes | es_ES |
dc.title | Development of new non-viral systems for genetic modification of senescent cells | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.access | info:eu-repo/semantics/openAccess | es_ES |
UDC.journalTitle | Molecular Therapy Nucleid Acids | es_ES |
UDC.volume | 32 | es_ES |
UDC.startPage | 302 | es_ES |
UDC.endPage | 317 | es_ES |
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