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HMGB1 Protein Interactions in Prostate and Ovary Cancer Models Reveal Links to RNA Processing and Ribosome Biogenesis through NuRD, THOC and Septin Complexes
dc.contributor.author | Barreiro-Alonso, Aida | |
dc.contributor.author | Lamas, Mónica | |
dc.contributor.author | Lorenzo Catoira, Lidia | |
dc.contributor.author | Pardo, Mercedes | |
dc.contributor.author | Yu, Lu | |
dc.contributor.author | Choudhary, Jyoti S. | |
dc.contributor.author | Cerdán, María Esperanza | |
dc.date.accessioned | 2021-12-22T12:09:31Z | |
dc.date.available | 2021-12-22T12:09:31Z | |
dc.date.issued | 2021-09-18 | |
dc.identifier.citation | Barreiro-Alonso, A.; Lamas-Maceiras, M.; Lorenzo-Catoira, L.; Pardo, M.; Yu, L.; Choudhary, J.S.; Cerdán, M.E. HMGB1 Protein Interactions in Prostate and Ovary Cancer Models Reveal Links to RNA Processing and Ribosome Biogenesis through NuRD, THOC and Septin Complexes. Cancers 2021, 13, 4686. https://doi.org/10.3390/cancers13184686 | es_ES |
dc.identifier.issn | 2072-6694 | |
dc.identifier.uri | http://hdl.handle.net/2183/29212 | |
dc.description.abstract | [Abstract] This study reports the HMGB1 interactomes in prostate and ovary cancer cells lines. Affinity purification coupled to mass spectrometry confirmed that the HMGB1 nuclear interactome is involved in HMGB1 known functions such as maintenance of chromatin stability and regulation of transcription, and also in not as yet reported processes such as mRNA and rRNA processing. We have identified an interaction between HMGB1 and the NuRD complex and validated this by yeast-two-hybrid, confirming that the RBBP7 subunit directly interacts with HMGB1. In addition, we describe for the first time an interaction between two HMGB1 interacting complexes, the septin and THOC complexes, as well as an interaction of these two complexes with Rab11. Analysis of Pan-Cancer Atlas public data indicated that several genes encoding HMGB1-interacting proteins identified in this study are dysregulated in tumours from patients diagnosed with ovary and prostate carcinomas. In PC-3 cells, silencing of HMGB1 leads to downregulation of the expression of key regulators of ribosome biogenesis and RNA processing, namely BOP1, RSS1, UBF1, KRR1 and LYAR. Upregulation of these genes in prostate adenocarcinomas is correlated with worse prognosis, reinforcing their functional significance in cancer progression. | es_ES |
dc.description.sponsorship | This research was funded by the Wellcome Trust (grant no. 206194/Z/17/Z) and by Plan Estatal I+D+i, Instituto Carlos III (ISCIII, Spain) (grants no. PI14/01031 and PI18/01417) cofunded by the Fondo Europeo de Desarrollo Regional-FEDER (The European Regional Development Fund-ERDF) “A way of Making Europe”, and by Xunta de Galicia (Consolidación Grupos Referencia Competitiva grant no. ED431C 2020-08) | es_ES |
dc.description.sponsorship | Reino Unido. Wellcome Trust; 206194/Z/17/Z | es_ES |
dc.description.sponsorship | Xunta de Galicia; ED431C 2020-08 | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | MDPI | es_ES |
dc.relation | info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/PI14%2F01031/ES/Interactoma de HMGB1 y HMGB2 y su relación con cáncer de ovario y próstata/ | |
dc.relation | info:eu-repo/grantAgreement/ISCIII/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PI18%2F01714/ES/Búsqueda de interacciones HMGB-IncRNAs útiles en diagnóstico cáncer de ovario/ | |
dc.relation.uri | https://doi.org/10.3390/cancers13184686 | es_ES |
dc.rights | Atribución 4.0 Internacional | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | HMGB1 interactome | es_ES |
dc.subject | Ribosome biogenesis | es_ES |
dc.subject | RNA processing | es_ES |
dc.subject | Ovary cancer | es_ES |
dc.subject | Prostate cancer | es_ES |
dc.title | HMGB1 Protein Interactions in Prostate and Ovary Cancer Models Reveal Links to RNA Processing and Ribosome Biogenesis through NuRD, THOC and Septin Complexes | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.access | info:eu-repo/semantics/openAccess | es_ES |
UDC.journalTitle | Cancers | es_ES |
UDC.volume | 13 | es_ES |
UDC.issue | 18 | es_ES |
UDC.startPage | 4686 | es_ES |
dc.identifier.doi | 10.3390/cancers13184686 |
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