Listar por autor "Fuentes Boquete, Isaac Manuel"
Mostrando ítems 21-40 de 53
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Differentiation of Synovial CD-105+ Human Mesenchymal Stem Cells into Chondrocyte-like Cells through Spheroid Formation
Arufe, M.C.; Fuente, Alexandre de la; Fuentes Boquete, Isaac Manuel; De-Toro, Javier; Blanco García, Francisco J (Wiley, 2009-06-18)[Abstract] Mesenchymal stem cells (MSCs) have the capacity to differentiate into several cell lineages, some of which can generate bone, cartilage, or adipose tissue. The presence of MSCs in the synovial membrane was ... -
Effects of severe hypoxia on bone marrow mesenchymal stem cells differentiation potential
Cicione, Claudia; Muiños-López, Emma; Hermida Gómez, Tamara; Fuentes Boquete, Isaac Manuel; Díaz-Prado, Silvia; Blanco García, Francisco J (Hindawi, 2013-06-30)[Abstract] Background. The interests in mesenchymal stem cells (MSCs) and their application in cell therapy have resulted in a better understanding of the basic biology of these cells. Recently hypoxia has been indicated ... -
Establishment of human induced pluripotent stem cell-lines (IPSC) for in vitro modelling hand ostheoarthritis.
Castro Viñuelas, Rocío; Sanjurjo-Rodríguez, Clara; Piñeiro-Ramil, María; Hermida Gómez, Tamara; Fuentes Boquete, Isaac Manuel; De-Toro, Javier; Blanco García, Francisco J; Díaz-Prado, Silvia (BMJ Journals, 2019-05-27) -
Generation and Characterization of Human Induced Pluripotent Stem Cells (iPSCs) From Hand Osteoarthritis Patient-Derived Fibroblasts
Castro Viñuelas, Rocío; Sanjurjo-Rodríguez, Clara; Piñeiro-Ramil, María; Hermida Gómez, Tamara; Rodríguez-Fernández, Silvia; Oreiro, Natividad; De-Toro, Javier; Fuentes Boquete, Isaac Manuel; Blanco García, Francisco J; Díaz-Prado, Silvia (Nature Research, 2020-03-06)[Abstract] Knowledge and research results about hand osteoarthritis (hOA) are limited due to the lack of samples and animal models of the disease. Here, we report the generation of two induced pluripotent stem cell ... -
Generation of a human control iPS cell line (ESi080‐A) from a donor with no rheumatic diseases
Castro Viñuelas, Rocío; Sanjurjo-Rodríguez, Clara; Piñeiro-Ramil, María; Rodríguez-Fernández, Silvia; Fuentes Boquete, Isaac Manuel; Blanco García, Francisco J; Díaz-Prado, Silvia (Elsevier, 2020-03)[Abstract] Here, we report the establishment of the human iPS cell line N1-FiPS4F#7 generated from skin cells of a patient with no rheumatic diseases, thus obtaining an appropriate control iPS cell line for researchers ... -
Generation of human immortalized chondrocytes from osteoarthritic and healthy cartilage
Piñeiro-Ramil, María; Sanjurjo-Rodríguez, Clara; Rodríguez-Fernández, Silvia; Hermida-Gómez, Tamara; Blanco García, Francisco J; Fuentes Boquete, Isaac Manuel; Vaamonde-García, Carlos; Díaz-Prado, Silvia (The British Editorial Society of Bone & Joint Surgery, 2023-01-17)[Abstract] Aims. After a few passages of in vitro culture, primary human articular chondrocytes undergo senescence and loss of their phenotype. Most of the available chondrocyte cell lines have been obtained from cartilage ... -
Generation of mesenchymal cell lines derived from aged donors
Piñeiro-Ramil, María; Sanjurjo-Rodríguez, Clara; Rodríguez-Fernández, Silvia; Castro Viñuelas, Rocío; Hermida Gómez, Tamara; Blanco García, Francisco J; Fuentes Boquete, Isaac Manuel; Díaz-Prado, Silvia (MDPI, 2021-10-01)[Abstract] Background: Mesenchymal stromal cells (MSCs) have the capacity for self-renewal and multi-differentiation, and for this reason they are considered a potential cellular source in regenerative medicine of cartilage ... -
Generation of osteoarthritic mesenchymal stromal cell lines.
Piñeiro-Ramil, María; Castro Viñuelas, Rocío; Sanjurjo-Rodríguez, Clara; Rodríguez-Fernández, Silvia; Hermida Gómez, Tamara; De-Toro, Javier; Blanco García, Francisco J; Fuentes Boquete, Isaac Manuel; Díaz-Prado, Silvia (BMJ Journals, 2019-05-27) -
Generation of osteoarthritis and healthy mesenchymal cell lines for research on regenerative medicine for osteoarthritis
Piñeiro-Ramil, María; Castro Viñuelas, Rocío; Sanjurjo-Rodríguez, Clara; Hermida Gómez, Tamara; Fuentes Boquete, Isaac Manuel; De-Toro, Javier; Blanco García, Francisco J; Díaz-Prado, Silvia (2018-04)[Purpose] Bone-marrow mesenchymal stem cells (BM-MSCs) are multipotent self-renewal adult cells with potential to regenerate the damaged tissues in degenerative diseases such as osteoarthritis (OA). Nevertheless, research ... -
Human amniotic membrane as an alternative source of stem cells for regenerative medicine
Díaz-Prado, Silvia; Muiños-López, Emma; Hermida Gómez, Tamara; Cicione, Claudia; Rendal Vázquez, María Esther; Fuentes Boquete, Isaac Manuel; De-Toro, Javier; Blanco García, Francisco J (Elsevier, 2011-02-19)[Abstract] The human amniotic membrane (HAM) is a highly abundant and readily available tissue. This amniotic tissue has considerable advantageous characteristics to be considered as an attractive material in the field of ... -
Human amniotic membrane: a potential tissue and cell source for cell therapy and regenerative medicine
Díaz-Prado, Silvia; Muíños-López, Emma; Fuentes Boquete, Isaac Manuel; De-Toro, Javier; Blanco García, Francisco J (Springer, 2013)[Abstract] The human amniotic membrane (HAM) is the innermost membrane surrounding the fetus. HAM is a highly abundant and readily available tissue that is becoming appreciated as an alternative to adult bone marrow ... -
Human amniotic mesenchymal stromal cells as favorable source for cartilage repair
Muiños-López, Emma; Hermida Gómez, Tamara; Fuentes Boquete, Isaac Manuel; De-Toro, Javier; Blanco García, Francisco J; Díaz-Prado, Silvia (Mary Ann Liebert, 2017-01-10)[Abstract] Introduction: Localized trauma-derived breakdown of the hyaline articular cartilage may progress toward osteoarthritis, a degenerative condition characterized by total loss of articular cartilage and joint ... -
Human cartilage engineering in an in vitro repair model using collagen scaffolds and mesenchymal stromal cells
Sanjurjo-Rodríguez, Clara; Castro Viñuelas, Rocío; Hermida Gómez, Tamara; Fuentes Boquete, Isaac Manuel; De-Toro, Javier; Blanco García, Francisco J; Díaz-Prado, Silvia (Ivyspring, 2017-09-28)[Abstract] The purpose of this study was to investigate cartilage repair of in vitro lesion models using human bone marrow mesenchymal stromal cells (hBMSCs) with different collagen (Col) scaffolds. Lesions were made ... -
Hydrogel-based localized nonviral gene delivery in regenerative medicine approaches: an overview
Carballo-Pedrares, Natalia; Fuentes Boquete, Isaac Manuel; Díaz-Prado, Silvia; Rey-Rico, Ana (MDPI, 2020-08-10)[Abstract] Hydrogel-based nonviral gene delivery constitutes a powerful strategy in various regenerative medicine scenarios, as those concerning the treatment of musculoskeletal, cardiovascular, or neural tissues disorders ... -
Immortalizing mesenchymal stromal cells from aged donors while keeping their essential features
Piñeiro-Ramil, María; Castro Viñuelas, Rocío; Sanjurjo-Rodríguez, Clara; Rodríguez-Fernández, Silvia; Hermida Gómez, Tamara; Blanco García, Francisco J; Fuentes Boquete, Isaac Manuel; Díaz-Prado, Silvia (Hindawi, 2020-06-16)[Abstract] Human bone marrow-derived mesenchymal stromal cells (MSCs) obtained from aged patients are prone to senesce and diminish their differentiation potential, therefore limiting their usefulness for osteochondral ... -
Induced pluripotent stem cells for cartilage repair: current status and future perspectives
Castro Viñuelas, Rocío; Sanjurjo-Rodríguez, Clara; Piñeiro-Ramil, María; Hermida Gómez, Tamara; Fuentes Boquete, Isaac Manuel; De-Toro, Javier; Blanco García, Francisco J; Díaz-Prado, Silvia (AO Research Institute Davos, 2018-09-11)[Abstract] The establishment of cartilage regenerative medicine is an important clinical issue, but the search for cell sources able to restore cartilage integrity proves to be challenging. Human mesenchymal stromal cells ... -
Influence of Age on Rat Bone-Marrow Mesenchymal Stem Cells Potential
Fafián Labora, Juan Antonio; Fernández-Pernas, Pablo; Fuentes Boquete, Isaac Manuel; De-Toro, Javier; Oreiro, Natividad; Sangiao-Alvarellos, Susana; Mateos, Jesús; Arufe, M.C. (Nature, 2015-11-19)[Abstract] Mesenchymal stem cells promising role in cell-based therapies and tissue engineering appears to be limited due to a decline of their regenerative potential with increasing donor age. Six age groups from bone ... -
Is Myofascial Release Therapy Cost-Effective When Compared with Manual Therapy to Treat Workers’ Mechanical Neck Pains?
Rodríguez Fuentes, Iván; De-Toro, Javier; Rodríguez Fuentes, Gustavo; Oliveira, Iris Machado de; Meijide-Faílde, Rosa; Fuentes Boquete, Isaac Manuel (Elsevier, 2020-09-12)[Abstract] Objective: The aim of this study was to do a cost-benefit analysis of myofascial release therapy (MRT) compared to manual therapy (MT) for treating occupational mechanical neck pain. Methods: Variables regarding ... -
Isolation and characterization of mesenchymal stem cells from human amniotic membrane
Díaz-Prado, Silvia; Muíños-López, Emma; Hermida Gómez, Tamara; Rendal Vázquez, María Esther; Fuentes Boquete, Isaac Manuel; De-Toro, Javier; Blanco García, Francisco J (Mary Ann Liebert, 2011-01-04)[Abstract] Introduction: The human amniotic membrane is a highly abundant and readily available tissue that may be useful for regenerative medicine and cell therapy. Aim: To compare two previously published protocols for ... -
Mesenchymal stem cells from human amniotic membrane
Sanjurjo-Rodríguez, Clara; Díaz-Prado, Silvia; Hermida-Gómez, Tamara; Fuentes Boquete, Isaac Manuel; Blanco García, Francisco J (Springer, 2014)[Abstract] The human amnion or amniotic membrane (HAM) has emerged as a novel and alternative source of stem cells. This tissue presents a reservoir of two types of stem cells from different embryological origins: human ...