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http://hdl.handle.net/2183/12848 Development of a reliable extraction method for the recovery of total genomic DNA from woodchip colonizing biofilm involved in gas biofiltration
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Authors
Cabrol, Léa
Malhautier, Luc
Rocher, Janick
Poly, Franck
Le Roux, Xavier
Jovic, Marc
Lepeuple, A.S.
Fanlo, Jean-Louis
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Journal Title
Bibliographic citation
Biotechniques for Air Pollution Control II, 2007: 517-528. ISBN: 978-84-9749-258-4
Type of academic work
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Abstract
[Abstract] This preliminary study focused on a critical step for the characterization of microbial ecosystem involved in biofiltration. Two aspects of nucleic acid recovery were explored: (i) cell dispersion (three methods tested) and (ii) total DNA extraction (four methods tested). The objective is to select the optimal combination of desorption/extraction methods, allowing subsequent molecular investigations to be reliable. Three relevant criteria are used to assess extraction efficiency: DNA amount and purity, and subsequent amplification feasibility.