Inter-laboratory evaluation of instrument platforms and experimental workflows for quantitative accuracy and reproducibility assessment

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UDC.coleccionInvestigación
UDC.departamentoFisioterapia, Medicina e Ciencias Biomédicas
UDC.endPage15
UDC.grupoInvReumatoloxía (INIBIC)
UDC.institutoCentroINIBIC - Instituto de Investigacións Biomédicas de A Coruña
UDC.journalTitleEuPA Open Proteomics
UDC.startPage6
UDC.volume8
dc.contributor.authorPercy, Andrew J.
dc.contributor.authorTamura-Wells, Jessica
dc.contributor.authorAlbar, Juan Pablo
dc.contributor.authorAloria, Kerman
dc.contributor.authorAmirkhani, Ardeshir
dc.contributor.authorAraújo, Gabriel D.T.
dc.contributor.authorArizmendi, Jesús M.
dc.contributor.authorBlanco García, Francisco J
dc.contributor.authorCanals, Francesc
dc.contributor.authorCho, Jin-Young
dc.contributor.authorColomé-Calls, Núria
dc.contributor.authorCorrales, Fernando J.
dc.contributor.authorDomont, Gilberto
dc.contributor.authorEspadas, Guadalupe
dc.contributor.authorFernández-Puente, Patricia
dc.contributor.authorGil, Concha
dc.contributor.authorHaynes, Paul A.
dc.contributor.authorHernáez, María Luisa
dc.contributor.authorKim, Jin Young
dc.contributor.authorKopylov, Arthur T.
dc.contributor.authorMarcilla, Miguel
dc.contributor.authorMcKay, Mathew J.
dc.contributor.authorMirzaei, Mehdi
dc.contributor.authorMolloy, Mark P.
dc.contributor.authorOhlund, Leanne B.
dc.contributor.authorPaik, Young-Ki
dc.contributor.authorParadela, Alberto
dc.contributor.authorRaftery, Mark
dc.contributor.authorSabidó, Eduard
dc.contributor.authorSleno, Lekha
dc.contributor.authorWilffert, Daniel
dc.contributor.authorWolters, Justina C.
dc.contributor.authorYoo, Jong Shin
dc.contributor.authorZgoda, Victor G.
dc.contributor.authorParker, Carol E.
dc.contributor.authorBorchers, Christoph H.
dc.date.accessioned2026-08-25T10:55:47Z
dc.date.available2026-08-25T10:55:47Z
dc.date.issued2015-06-11
dc.description.abstract[Abstract] The reproducibility of plasma protein quantitation between laboratories and between instrument types was examined in a large-scale international study involving 16 laboratories and 19 LC-MS/MS platforms, using two kits designed to evaluate instrument performance and one kit designed to evaluate the entire bottom-up workflow. There was little effect of instrument type on the quality of the results, demonstrating the robustness of LC/MRM-MS with isotopically labeled standards. Technician skill was a factor, as errors in sample preparation and sub-optimal LC-MS performance were evident. This highlights the importance of proper training and routine quality control before quantitation is done on patient samples.
dc.description.sponsorshipThe UVic-Genome BC Proteomics Centre would like to thank Genome Canada and Genome British Columbia for STIC funding and support, as well as Western Economic Diversification of Canada for instrumentation support. All laboratories from Spain are members of ProteoRed (Plataforma de Recursos Biomoleculares y Bioinformáticos) and are supported by grant PT13/0001 funded by Instituto de Salud Carlos III (ISCIII) and the European Regional Development Fund (FEDER). FC wishes to acknowledge support from Grant PRB2 (IPT13/0001 - ISCIII-SGEFI/FEDER). GD wishes to acknowledge support from grants FAPERJ E26-110.138/2013, FAPERJ E-26/111.697/2013, and FAPERJ E-26/0010.1582/2014. AK and VZ state that the Russian portion of the work was done in the framework of the State Academies Fundamental Research Program (2013-2020). Reynaldo Interior (Hospital for Sick Children; Toronto, ON, Canada), Jay Gambee (AAA Service Laboratory; Damascus, OR, USA), and David Chen (University of British Columbia; Vancouver, BC, Canada) are recognized for characterizing the 43 SIS peptides. PAH wishes to thank Ardeshir Amirkhani of the Australian Proteome Analysis Facility, Macquarie University, Sydney, Australia for assistance with sample preparation and data analysis.
dc.identifier.citationPercy AJ, Tamura-Wells J, Albar JP, Aloria K, Amirkhani A, Araujo GD, et al. Inter-laboratory evaluation of instrument platforms and experimental workflows for quantitative accuracy and reproducibility assessment. EuPA Open Proteom. 2015 Sep;8:6-15. DOI: 10.1016/j.euprot.2015.06.001
dc.identifier.doi10.1016/J.EUPROT.2015.06.001
dc.identifier.issn2212-9685
dc.identifier.urihttps://hdl.handle.net/2183/49089
dc.language.isoeng
dc.publisherElsevier
dc.relation.projectIDinfo:eu-repo/grantAgreement/MINECO//PT13%2F0001%2F0012/ES/Plataforma de recursos biomoleculares y bioinformaticos/
dc.relation.urihttps://doi.org/10.1016/J.EUPROT.2015.06.001
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internationalen
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectAccuracy
dc.subjectHuman plasma
dc.subjectQuality control
dc.subjectQuantitative proteomics
dc.subjectReproductibility
dc.subjectWorkflow validation
dc.titleInter-laboratory evaluation of instrument platforms and experimental workflows for quantitative accuracy and reproducibility assessment
dc.typejournal article
dc.type.hasVersionVoR
dspace.entity.typePublication
relation.isAuthorOfPublicationf357279a-035a-4279-a553-99cfd79bd2bb
relation.isAuthorOfPublication.latestForDiscoveryf357279a-035a-4279-a553-99cfd79bd2bb

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