Inter-laboratory evaluation of instrument platforms and experimental workflows for quantitative accuracy and reproducibility assessment

Percy, Andrew J.; Tamura-Wells, Jessica; Albar, Juan Pablo; Aloria, Kerman; Amirkhani, Ardeshir; Araújo, Gabriel D.T.; Arizmendi, Jesús M.; Blanco García, Francisco J; Canals, Francesc; Cho, Jin-Young; Colomé-Calls, Núria; Corrales, Fernando J.; Domont, Gilberto; Espadas, Guadalupe; Fernández-Puente, Patricia; Gil, Concha; Haynes, Paul A.; Hernáez, María Luisa; Kim, Jin Young; Kopylov, Arthur T.; Marcilla, Miguel; McKay, Mathew J.; Mirzaei, Mehdi; Molloy, Mark P.; Ohlund, Leanne B.; Paik, Young-Ki; Paradela, Alberto; Raftery, Mark; Sabidó, Eduard; Sleno, Lekha; Wilffert, Daniel; Wolters, Justina C.; Yoo, Jong Shin; Zgoda, Victor G.; Parker, Carol E.; Borchers, Christoph H.

Use this link to cite:

https://hdl.handle.net/2183/49089

Inter-laboratory evaluation of instrument platforms and experimental workflows for quantitative accuracy and reproducibility assessment

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Identifiers

URI: https://hdl.handle.net/2183/49089

DOI: 10.1016/J.EUPROT.2015.06.001

Publication date

2015-06-11

Authors

Percy, Andrew J.

Tamura-Wells, Jessica

Albar, Juan Pablo

Aloria, Kerman

Amirkhani, Ardeshir

Araújo, Gabriel D.T.

Arizmendi, Jesús M.

Blanco García, Francisco J

Canals, Francesc

Cho, Jin-Young

Bibliographic citation

Percy AJ, Tamura-Wells J, Albar JP, Aloria K, Amirkhani A, Araujo GD, et al. Inter-laboratory evaluation of instrument platforms and experimental workflows for quantitative accuracy and reproducibility assessment. EuPA Open Proteom. 2015 Sep;8:6-15. DOI: 10.1016/j.euprot.2015.06.001

Abstract

[Abstract] The reproducibility of plasma protein quantitation between laboratories and between instrument types was examined in a large-scale international study involving 16 laboratories and 19 LC-MS/MS platforms, using two kits designed to evaluate instrument performance and one kit designed to evaluate the entire bottom-up workflow. There was little effect of instrument type on the quality of the results, demonstrating the robustness of LC/MRM-MS with isotopically labeled standards. Technician skill was a factor, as errors in sample preparation and sub-optimal LC-MS performance were evident. This highlights the importance of proper training and routine quality control before quantitation is done on patient samples.