Two-stage syngas fermentation into microbial oils and β-carotene with Clostridium carboxidivorans and engineered Yarrowia lipolytica
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Two-stage syngas fermentation into microbial oils and β-carotene with Clostridium carboxidivorans and engineered Yarrowia lipolyticaAutor(es)
Fecha
2023Cita bibliográfica
Robles-Iglesias, R., Naveira-Pazos, C., Nicaud, J.-M., Veiga, M. C., & Kennes, C. (2023). Two-stage syngas fermentation into microbial oils and β-carotene with Clostridium carboxidivorans and engineered Yarrowia lipolytica. Journal of CO2 Utilization, 76. https://doi.org/10.1016/J.JCOU.2023.102593
Resumen
[Abstract] An engineered Yarrowia lipolytica strain was able to simultaneously accumulate microbial oils and β-carotene from both acetic and butyric acids produced from syngas fermentation by Clostridium carboxidivorans, through the Wood-Ljungdahl pathway, with CO and CO2 as carbon sources. It was first determined that Y. lipolytica showed no inhibition at concentrations < 16 g/L of a mixture of VFAs (Volatile Fatty Acids) containing acetic, butyric, and even hexanoic acids. The yeast was then grown in a bioreactor with a culture medium derived from acetogenic syngas (CO, CO2, H2) fermentation containing all three acids. Y. lipolytica consumed a total of 91 g/L VFAs, progressively supplied through eight successive additions of these acids to the reactor. A maximum lipid content of 36.18% g/g cell was achieved at 61 g/L VFAs consumed and with an airflow rate of 2.0 vvm. Besides, a total of 88.4 mg/g cell and 759 mg/L β-carotene content and concentration were achieved at about 80 g/L VFAs consumed and with a 2.0 vvm airflow rate. It was concluded that; a non-limiting air flow favors the metabolic pathway for β-carotene formation against lipid accumulation
Palabras clave
Acetogen
Carbon dioxide
Carbon monoxide
Lipids
Oleaginous yeast
Carbon dioxide
Carbon monoxide
Lipids
Oleaginous yeast
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Financiado para publicación en acceso aberto: Universidade da Coruña/CISUG
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CC BY-NC-ND 4.0 Attribution-NonCommercial-NoDerivatives 4.0 International