Suitability of the In Vitro Cytokinesis-Block Micronucleus Test for Genotoxicity Assessment of TiO2 Nanoparticles on SH-SY5Y Cells
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Suitability of the In Vitro Cytokinesis-Block Micronucleus Test for Genotoxicity Assessment of TiO2 Nanoparticles on SH-SY5Y CellsAutor(es)
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2021Cita bibliográfica
Fernández-Bertólez, N.; Brandão, F.; Costa, C.; Pásaro, E.; Teixeira, J.P.; Laffon, B.; Valdiglesias, V. Suitability of the In Vitro Cytokinesis-Block Micronucleus Test for Genotoxicity Assessment of TiO2 Nanoparticles on SH-SY5Y Cells. Int. J. Mol. Sci. 2021, 22, 8558. https:// doi.org/10.3390/ijms22168558
Resumen
[Abstract] Standard toxicity tests might not be fully adequate for evaluating nanomaterials since
their unique features are also responsible for unexpected interactions. The in vitro cytokinesis-block
micronucleus (CBMN) test is recommended for genotoxicity testing, but cytochalasin-B (Cyt-B) may
interfere with nanoparticles (NP), leading to inaccurate results. Our objective was to determine
whether Cyt-B could interfere with MN induction by TiO2 NP in human SH-SY5Y cells, as assessed
by CBMN test. Cells were treated for 6 or 24 h, according to three treatment options: co-treatment
with Cyt-B, post-treatment, and delayed co-treatment. Influence of Cyt-B on TiO2 NP cellular uptake
and MN induction as evaluated by flow cytometry (FCMN) were also assessed. TiO2 NP were
significantly internalized by cells, both in the absence and presence of Cyt-B, indicating that this
chemical does not interfere with NP uptake. Dose-dependent increases in MN rates were observed in
CBMN test after co-treatment. However, FCMN assay only showed a positive response when Cyt-B
was added simultaneously with TiO2 NP, suggesting that Cyt-B might alter CBMN assay results. No
differences were observed in the comparisons between the treatment options assessed, suggesting
they are not adequate alternatives to avoid Cyt-B interference in the specific conditions tested.
Palabras clave
Cytochalasin-B
Cytokinesis-block micronucleus test
Flow cytometry micronucleus test
Nanomaterials
TiO2 nanoparticles
SH-SY5Y cells
Uptake
Cytokinesis-block micronucleus test
Flow cytometry micronucleus test
Nanomaterials
TiO2 nanoparticles
SH-SY5Y cells
Uptake
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Atribución 4.0 Internacional