Fuentes-Monteverde, Juan CarlosNúñez, Marvin J.Amaya, ÓscarMartínez, Morena L.Rodríguez, JaimeJiménez, Carlos2024-06-262024-06-262023-06-25Fuentes-Monteverde, J.C.; Núñez, M.J.; Amaya-Monterosa, O.; Martínez, M.L.; Rodríguez, J.; Jiménez, C. Multistage Detection of Tetrodotoxin Traces in Diodon Hystrix Collected in El Salvador. Toxins 2023, 15, 409, doi:10.3390/toxins15070409.2072-6651http://hdl.handle.net/2183/37420[Abstract] This study describes a multistage methodology to detect minute amounts of tetrodotoxin in fishes, a plan that may be broadened to include other marine organisms. This methodology was applied to porcupinefish (Diodon hystrix) collected in Punta Chiquirín, El Salvador. A three-stage approach along with post-acquisition processing was employed, to wit: (a) Sample screening by selected reaction monitoring (HPLC-MS/MS-SRM) analyses to quickly identify possible toxin presence via a LC/MS/MS API 3200 system with a triple quadrupole; (b) HPLC-HRFTMS-full scan analyses using an ion trap-Orbitrap spectrometer combined with an MZmine 2-enhanced dereplication-like workflow to collect high-resolution mass spectra; and (c) HPLC-HRMS2 analyses. This is the first time tetrodotoxin has been reported in D. hystrix specimens collected in El Salvador.engAtribución 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/TetrodotoxinHPLC-HRMSHPLC-MS/MS-SRMHPLC-HRMS2Diodon hystrixMZmineNatural products detectionjournal articleopen access10.3390/toxins15070409