Conde-Pérez, KellyBuetas, ElenaAja-Macaya, PabloMartin-De Arribas, E.Iglesias Corrás, IagoTrigo Tasende, NoeliaNasser-Ali, MohammedEstévez, LaraRumbo-Feal, SorayaOtero Alén, BegoñaNoguera, José F.Concha López, ÁngelPardiñas López, SimónCarda-Diéguez, MiguelGómez-Randulfe, IgorMartínez-Lago, NievesLadra, SusanaAntón-Aparicio, Luis M.Bou, GermánMira, AlexVallejo, J. A.Poza, Margarita2024-09-032024-09-032023-08-09Conde-Pérez, K., Buetas, E., Aja-Macaya, P., Martin-De Arribas, E., Iglesias-Corrás, I., Trigo-Tasende, N., Nasser-Ali, M., Estévez, L. S., Rumbo-Feal, S., Otero-Alén, B., Noguera, J. F., Concha, Á., Pardiñas-López, S., Carda-Diéguez, M., Gómez-Randulfe, I., Martínez-Lago, N., Ladra, S., Aparicio, L. A., Bou, G., et al. (2023). Parvimonas micra can translocate from the subgingival sulcus of the human oral cavity to colorectal adenocarcinoma. Molecular Oncology. 18(5). 1143-1173 https://doi.org/10.1002/1878-0261.13506http://hdl.handle.net/2183/38829[Abstract] Oral and intestinal samples from a cohort of 93 colorectal cancer (CRC) patients and 30 healthy controls (non-CRC) were collected for microbiome analysis. Saliva (28 non-CRC and 94 CRC), feces (30 non-CRC and 97 CRC), subgingival fluid (20 CRC), and tumor tissue samples (20 CRC) were used for 16S metabarcoding and/or RNA sequencing (RNAseq) approaches. A differential analysis of the abundance, performed with the ANCOM-BC package, adjusting the P-values by the Holm-Bonferroni method, revealed that Parvimonas was significantly over-represented in feces from CRC patients (P-value < 0.001) compared to healthy controls. A total of 11 Parvimonas micra isolates were obtained from the oral cavity and adenocarcinoma of CRC patients. Genome analysis identified a pair of isolates from the same patient that shared 99.2% identity, demonstrating that P. micra can translocate from the subgingival cavity to the gut. The data suggest that P. micra could migrate in a synergistic consortium with other periodontal bacteria. Metatranscriptomics confirmed that oral bacteria were more active in tumor than in non-neoplastic tissues. We suggest that P. micra could be considered as a CRC biomarker detected in non-invasive samples such as feces.engAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/Colorectal cancerMetabarcodingMetatranscriptomicsMicrobiomeParvimonas micraPeriodontal diseasejournal articleopen access10.1002/1878-0261.13506