Wetzl, CeciliaBrosel, SergiCarini, MarcoDi Silvio, DesiréIlla, XaviVilla, RosaGuimerà, AntonPrats Alfonso, ElisabetPrato, MaurizioCriado, AlejandroRoyal Society of Chemistry2024-06-212024-06-212023-09-19C. Wetzl, S. Brosel-Oliu, M. Carini, D. Di Silvio, X. Illa, R. Villa, A. Guimera, E. Prats-Alfonso, M. Prato and A. Criado, Nanoscale, 2023, DOI: 10.1039/d3nr04153k2040-3372http://hdl.handle.net/2183/37305[Abastract]: In the last decade, solution-gated graphene field effect transistors (GFETs) showed their versatility in the development of a miniaturized multiplexed platform for electrophysiological recordings and sensing. Due to their working mechanism, the surface functionalisation and immobilisation of receptors are pivotal to ensure the proper functioning of devices. Herein, we present a controlled covalent functionalisation strategy based on molecular design and electrochemical triggering, which provide a monolayer-like functionalisation of micro-GFET arrays retaining the electronic properties of graphenes. The functionalisation layer as a receptor was then employed as the linker for serotonin aptamer conjugation. The micro-GFET arrays display sensitivity toward the target analyte in the micromolar range in a physiological buffer (PBS 10 mM). The sensor allows the in-flow real-time monitoring of serotonin transient concentrations with fast and reversible responses.engThis journal is © The Royal Society of Chemistry 2023. This article is licensed under a Creative Commons Attribution 3.0 Unported Licence http://creativecommons.org/licenses/by/3.0/NanoscaleChemistryGrowthFilmsGraphiteSerotoninBiosensing techniquesTransistorsAptamersjournal articleopen access10.1039/D3NR04153K