Volkmer, TiagoMagalhães, JoanaSousa, VaniaSantos, Luis A.Burguera, Elena F.Blanco García, Francisco JSan Román, JulioRodríguez-Lorenzo, Luis M.2025-05-162025-05-162014-09-29Volkmer T, Magalhaes J, Sousa V, Santos LA, Burguera EF, Blanco FJ, San Román J, Rodríguez-Lorenzo LM. 2-(dimethylamino)ethyl methacrylate/(2-hydroxyethyl) methacrylate/α-tricalcium phosphate cryogels for bone repair, preparation and evaluation of the biological response of human trabecular bone-derived cells and mesenchymal stem cells. Polymers (Basel). 2014; 6(10):2510-2525.2073-4360http://hdl.handle.net/2183/42009[Abstract] The aim of this work is to evaluate the potential of cryogels to be used as scaffolds in tissue engineering. Scaffolds based on the α-tricalcium phosphate reinforced PDMAEMA (Poly(dimethyl aminoethyl methacrylate))/PHEMA (poly(hydroxyethyl methacrylate)) system were prepared and human trabecular bone-derived cells (HTBs) and bone marrow derived-mesenchymal stem cells (BM-MSCs) cultured on them. Several features, such as porosity, pore shape, molecular weight between crosslinks and mesh size, are studied. The most suitable PDMAEMA/PHEMA ratio for cell proliferation has been assessed and the viability, adhesion, proliferation and expression of osteoblastic biochemical markers are evaluated. The PDMAEMA/PHEMA ratio influences the scaffolds porosity. Values between 53% ± 5.7% for a greater content in PHEMA and 75% ± 5.5% for a greater content in PDMAEMA have been obtained. The polymer ratio also modifies the pore shape. A greater content in PDMAEMA leads also to bigger network mesh size. Each of the compositions were non-cytotoxic, the seeded cells remained viable for both BM-MSCs and HTBs. Thus, and based on the structural analysis, specimens with a greater content in PDMAEMA seem to provide a better structural environment for their use as scaffolds for tissue engineering. The α-tricalcium phosphate incorporation into the composition seems to favor the expression of the osteogenic phenotype.engCreative Commons Attribution 4.0 International License (CC-BY 4.0)http://creativecommons.org/licenses/by/3.0/es/Cryogelsα-tricalcium phosphatePDMAEMA (Poly(dimethyl aminoethyl methacrylate))PHEMA (poly(hydroxyethyl methacrylate)Mesenchymal stem cellsjournal articleopen access10.3390/polym6102510