Cabrol, LéaMalhautier, LucRocher, JanickPoly, FranckLe Roux, XavierJovic, MarcLepeuple, A.S.Fanlo, Jean-Louis2014-10-012014-10-012007Biotechniques for Air Pollution Control II, 2007: 517-528. ISBN: 978-84-9749-258-4978-84-9749-258-4http://hdl.handle.net/2183/12848[Abstract] This preliminary study focused on a critical step for the characterization of microbial ecosystem involved in biofiltration. Two aspects of nucleic acid recovery were explored: (i) cell dispersion (three methods tested) and (ii) total DNA extraction (four methods tested). The objective is to select the optimal combination of desorption/extraction methods, allowing subsequent molecular investigations to be reliable. Three relevant criteria are used to assess extraction efficiency: DNA amount and purity, and subsequent amplification feasibility.engconference outputopen access