Bellas, JuanRial, DiegoValdés, JulianaVidal‑Liñán, LeticiaBertucci, Juan I.Muniategui, SoledadLeón, Víctor M.Campillo, Juan Antonio2025-04-112025-04-112022-02-14Bellas, J., Rial, D., Valdés, J. et al. Linking biochemical and individual-level effects of chlorpyrifos, triphenyl phosphate, and bisphenol A on sea urchin (Paracentrotus lividus) larvae. Environ Sci Pollut Res 29, 46174–46187 (2022).0944-1344http://hdl.handle.net/2183/41720[Abstract] The effects of three relevant organic pollutants: chlorpyrifos (CPF), a widely used insecticide, triphenyl phosphate (TPHP), employed as flame retardant and as plastic additive, and bisphenol A (BPA), used primarily as plastic additive, on sea urchin (Paracentrotus lividus) larvae, were investigated. Experiments consisted of exposing sea urchin fertilized eggs throughout their development to the 4-arm pluteus larval stage. The antioxidant enzymes glutathione reductase (GR) and catalase (CAT), the phase II detoxification enzyme glutathione S-transferase (GST), and the neurotransmitter catabolism enzyme acetylcho-linesterase (AChE) were assessed in combination with responses at the individual level (larval growth). CPF was the most toxic compound with 10 and 50% effective concentrations (EC 10 and EC 50 ) values of 60 and 279 μg/l (0.17 and 0.80 μM), followed by TPHP with EC 10 and EC 50 values of 224 and 1213 μg/l (0.68 and 3.7 μM), and by BPA with EC 10 and EC 50 values of 885 and 1549 μg/l (3.9 and 6.8 μM). The toxicity of the three compounds was attributed to oxidative stress, to the modulation of the AChE response, and/or to the reduction of the detoxification efficacy. Increasing trends in CAT activity were observed for BPA and, to a lower extent, for CPF. GR activity showed a bell-shaped response in larvae exposed to CPF, whereas BPA caused an increasing trend in GR. GST also displayed a bell-shaped response to CPF exposure and a decreas-ing trend was observed for TPHP. An inhibition pattern in AChE activity was observed at increasing BPA concentrations. A potential role of the GST in the metabolism of CPF was proposed, but not for TPHP or BPA, and a significant increase of AChE activity associated with oxidative stress was observed in TPHP-exposed larvae. Among the biochemical responses, the GR activity was found to be a reliable biomarker of exposure for sea urchin early-life stages, providing a first sign of damage. These results show that the integration of responses at the biochemical level with fitness-related responses (e.g., growth) may help to improve knowledge about the impact of toxic substances on marine ecosystems.engAtribución 3.0 Españahttp://creativecommons.org/licenses/by/3.0/es/AcetylcholinesteraseAntioxidant enzymesBiomarkersEmbryo-larval bioassayGlutathione S-transferaseSea urchinjournal articleopen access