Magalhães, JoanaCrawford, AileenHatton, Paul V.Blanco García, Francisco JSan Román, Julio2026-09-232026-09-232014-10-31Magalhães J, Crawford A, Hatton PV, Blanco FJ, Roman JS. Poly(2-ethyl-(2-pyrrolidone) methacrylate) and hyaluronic acid–based hydrogels for the engineering of a cartilage-like tissue using bovine articular chondrocytes. Journal of Bioactive and Compatible Polymers. 2014;29(6):545-559. doi:10.1177/08839115145556091530-8030https://hdl.handle.net/2183/49386[Abstract] Poly(2-ethyl-(2-pyrrolidone)methacrylate)–hyaluronic acid hydrogels based on the free radical polymerization of 2-ethyl-(2-pyrrolidone)methacrylate combined with hyaluronic acid, using N,N′-methylenebisacrylamide or triethylene glycol dimethacrylate, as cross-linking agents, were considered for tissue engineering applications. Bovine articular chondrocytes were seeded onto the poly(2-ethyl-(2-pyrrolidone)methacrylate)–hyaluronic acid hydrogels, under orbital agitation, for a total of 40 days. The engineered cell-constructs were characterized according to cell proliferation, morphology and distribution as well as the biochemical composition of the tissue formed. The chondrocytes were found to be attached and presented a typical spherical morphology. Cells were able to proliferate and synthesize a hyaline-like matrix rich in glycosaminoglycans and collagen type II which were mainly located on the superficial area. Increased content of individual components poly(2-ethyl-(2-pyrrolidone)methacrylate) and hyaluronic acid, in triethylene glycol dimethacrylate–cross-linked networks led to enhanced cell distribution and total glycosaminoglycans content, supporting their potential application for the repair of cartilaginous tissues.engHydrogelsHyaluronic acidBiocompatibilityChondrocytesCartilage tissue engineeringjournal articleopen access10.1177/0883911514555609