Cabral, M. P.Soares, Nelson C.Aranda Rodríguez, JesúsParreira, José RicardoRumbo, CarlosPoza, MargaritaValle, JaioneCalamia, ValentinaLasa, ÍñigoBou, Germán2025-09-222025-09-222011-05-26Cabral MP, Soares NC, Aranda J, Parreira JR, Rumbo C, Poza M, Valle J, Calamia V, Lasa I, Bou G. Proteomic and functional analyses reveal a unique lifestyle for Acinetobacter baumannii biofilms and a key role for histidine metabolism. J Proteome Res. 2011 Aug 5;10(8):3399-417.1535-3893https://hdl.handle.net/2183/45795[Abstract] Biofilm formation is one of the main causes for the persistence of Acinetobacter baumannii, a pathogen associated with severe infections and outbreaks in hospitals. Here, we performed comparative proteomic analyses (2D-DIGE and MALDI-TOF/TOF and iTRAQ/SCX-LC-MS/MS) of cells at three different conditions: exponential, late stationary phase, and biofilms. These results were compared with alterations in the proteome resulting from exposure to a biofilm inhibitory compound (salicylate). Using this multiple-approach strategy, proteomic patterns showed a unique lifestyle for A. baumannii biofilms and novel associated proteins. Several cell surface proteins (such as CarO, OmpA, OprD-like, DcaP-like, PstS, LysM, and Omp33), as well as those involved in histidine metabolism (like Urocanase), were found to be implicated in biofilm formation, this being confirmed by gene disruption. Although l-His uptake triggered biofilms efficiently in wild-type A. baumannii, no effect was observed in Urocanase and OmpA mutants, while a slight increase was observed in a CarO deficient strain. We conclude that Urocanase plays a crucial role in histidine metabolism leading to biofilm formation and that OmpA and CarO can act as channels for L-His uptake. Finally, we propose a model in which novel proteins are suggested for the first time as targets for preventing the formation of A. baumannii biofilms.engThis document is the Accepted Manuscript version of a Published Work that appeared in final form in Journal of Proteome Research, copyright © 2011 American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see ACS PUblications web page.ProteomicsAcinetobacter baumanniiBiofilmsHistidineSalycilate2DEiTRAQMALDI-TOF/TOFjournal articleopen access10.1021/pr101299j