Bermúdez, María JoséEiro, NoemiSendón Lago, Juan JoséCid, SandraSaa, JorgePablo, Nagore deVega, BelenPérez-Fernández, RománVizoso, Francisco2024-08-292024-08-292022Eiro, N., Sendon-Lago, J., Cid, S., Saa, J., de Pablo, N., Vega, B., Bermudez, M. A., Perez-Fernandez, R., & Vizoso, F. J. (2022). Conditioned Medium from Human Uterine Cervical Stem Cells Regulates Oxidative Stress and Angiogenesis of Retinal Pigment Epithelial Cells. Ophthalmic research, 65(5), 556-565. https://doi.org/10.1159/000524484http://hdl.handle.net/2183/38756[Abstract] Introduction: Retinal homeostasis is essential to avoid retinal pigment epithelium (RPE) damage resulting in photoreceptor death and blindness. Mesenchymal stem cells-based cell therapy could contribute to the maintenance of the retinal homeostasis. We have explored the effect of human uterine cervical stem cells (hUCESCs)-conditioned medium (hUCESC-CM) on RPE cells under oxidative stress condition. Methods: ARPE-19 cells were treated with hydrogen peroxide (H2O2) in the presence or absence of hUCESC-CM. qRT-PCR and Western blot were used to evaluate the expression of oxidative stress-related (HO-1, GCLC, and HSPB1) and vasculogenesis-related (VEGFA, PDGFA, and PDGFB) factors. Also, we assessed in vitro effects of hUCESC-CM on endothelial-cell (HUVEC) tube formation. Results: mRNA expression of HO-1, GCLC, HSPB1, VEGFA, PDGFA, and PDGFB were significantly increased in ARPE-19 cells treated with H2O2 + hUCESC-CM compared to cells treated with H2O2 only. Regarding the tube formation assay, HUVEC treated with supernatant from ARPE-19 cells treated with H2O2 + hUCESC-CM showed a significant increase in average vessel length, number of capillary-like junctions, and average of vessels area compared with HUVEC treated with supernatant from ARPE-19 cells treated with H2O2 only. Conclusion: Our results show potential therapeutic effects of hUCESC-CM on RPE, such as protection from damage by oxidative stress, stimulation of detoxifying genes, and a better vascularization.engCreative Commons Attribution-NonCommercial-4.0 International License (CC BY-NC)http://creativecommons.org/licenses/by-nc/3.0/es/Mesenchymal stem cellsHuman uterine cervical stem cellsConditioned mediumSecretomeRetinal pigment epitheliumOxidative stressAngiogenesisjournal articleopen access10.1159/000524484