Robles-Iglesias, RaulVeiga, María CarmenKennes, ChristianNicaud, Jean-Marc2024-08-092024-08-092023Robles-Iglesias, R., Nicaud, J.-M., Veiga, M. C., & Kennes, C. (2023). Integrated fermentative process for lipid and β-carotene production from acetogenic syngas fermentation using an engineered oleaginous Yarrowia lipolytica yeast. Bioresource Technology, 389. https://doi.org/10.1016/J.BIORTECH.2023.129815http://hdl.handle.net/2183/38513Financiado para publicación en acceso aberto: Universidade da Coruña/CISUG[Abstract] An engineered Yarrowia lipolytica strain was successfully employed to produce β-carotene and lipids from acetic acid, a product of syngas fermentation by Clostridium aceticum. The strain showed acetic acid tolerance up to concentrations of 20 g/L. Flask experiments yielded a peak lipid content of 33.7 % and β-carotene concentration of 13.6 mg/g under specific nutrient conditions. The study also investigated pH effects on production in bioreactors, revealing optimal lipid and β-carotene contents at pH 6.0, reaching 22.9 % and 44 mg/g, respectively. Lipid profiles were consistent across experiments, with C18:1 being the dominant compound at approximately 50 %. This research underscores a green revolution in bioprocessing, showing how biocatalysts can convert syngas, a potentially polluting byproduct, into valuable β-carotene and lipids with a Y. lipolytica strain.engCC BY-NC-ND 4.0 Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/Acetogenic bacteriaBiodieselCarbon dioxideβ-caroteneMicrobial oilSustainable fueljournal articleopen access10.1016/j.biortech.2023.129815