Disorders provoked by copper in the marine diatom Phaeodactylum tricornutum in short-time exposure asays

Abstract

[Abstract] Copper toxicity on the marine diatom Phaeodactylwn tricomutum was assessed after 24.liours of exposure, using different parameters: growth, 14C-bicarbonate assimilation, 3H-thymidine incorporation, photosynthetic pigment content, and others assayed by flow cytometry, such as cellular volume, chlorophyll a autofluorescence and cell viability. Different degrees of sensitivity were observed for the different analysis. Cell viability assayed by flow cytometry was the less sensitive parameter.[Resume] La toxicite du cuivre sur la diatomee marine Phaeodactylum tricomutum. Cette toxicite a ete etudiee apres 24 heures d'exposition, par la mesure de differents parametres: croissance, assimilation de 14C-bicarbonate, incorporation de 3H-thymidine, teneur en pigment photosynthetique, et d'autres parametres evalues par cytometrie de flux, tels que Ie volume cellulaire, l' autofluorescence de la chlorophylle a et la viabilite cellulaire. Differents degres de sensibilite ont ete observes pour les differentes analyses. La viabilite cellulaire, etudiee par cytometrie de flux, s'est averee comme Ie parametre Ie moins sensible.