Multistage Detection of Tetrodotoxin Traces in Diodon hystrix Collected in El Salvador

Fuentes-Monteverde, Juan Carlos; Núñez, Marvin J.; Amaya, Óscar; Martínez, Morena L.; Rodríguez, Jaime; Jiménez, Carlos

Use this link to cite:

http://hdl.handle.net/2183/37420

Multistage Detection of Tetrodotoxin Traces in Diodon hystrix Collected in El Salvador

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Jimenez_Carlos_2023_Multistage_detection_tetrodotoxin_traces_diodon_hystrix.pdf (3.95 MB)

Identifiers

URI: http://hdl.handle.net/2183/37420

DOI: 10.3390/toxins15070409

Publication date

2023-06-25

Authors

Fuentes-Monteverde, Juan Carlos

Núñez, Marvin J.

Amaya, Óscar

Martínez, Morena L.

Rodríguez, Jaime

Jiménez, Carlos

Bibliographic citation

Fuentes-Monteverde, J.C.; Núñez, M.J.; Amaya-Monterosa, O.; Martínez, M.L.; Rodríguez, J.; Jiménez, C. Multistage Detection of Tetrodotoxin Traces in Diodon Hystrix Collected in El Salvador. Toxins 2023, 15, 409, doi:10.3390/toxins15070409.

Abstract

[Abstract] This study describes a multistage methodology to detect minute amounts of tetrodotoxin in fishes, a plan that may be broadened to include other marine organisms. This methodology was applied to porcupinefish (Diodon hystrix) collected in Punta Chiquirín, El Salvador. A three-stage approach along with post-acquisition processing was employed, to wit: (a) Sample screening by selected reaction monitoring (HPLC-MS/MS-SRM) analyses to quickly identify possible toxin presence via a LC/MS/MS API 3200 system with a triple quadrupole; (b) HPLC-HRFTMS-full scan analyses using an ion trap-Orbitrap spectrometer combined with an MZmine 2-enhanced dereplication-like workflow to collect high-resolution mass spectra; and (c) HPLC-HRMS2 analyses. This is the first time tetrodotoxin has been reported in D. hystrix specimens collected in El Salvador.