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http://hdl.handle.net/2183/39817

Deactivation of a dimeric DNA-binding peptide through a palladium-mediated self-immolative cleavage

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Identifiers

URI: http://hdl.handle.net/2183/39817
DOI: 10.1039/D1RA09180H

Publication date

2022-01-26

Authors

Pérez-González, Daniel Cibrán
Martínez-Calvo, Miguel
Mascareñas, José

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Bibliographic citation

RSC Adv., 2022, 12, 3500

Type of academic work

Academic degree

Abstract

[Abstract]: Herein, we describe an approach for the on-demand disassembly of dimeric peptides using a palladium-mediated cleavage of a designed self-immolative linker. The utility of the strategy is demonstrated for the case of dimeric basic regions of bZIP transcription factors. While the dimer binds designed DNA sequences with good affinities, the peptide–DNA complex can be readily dismounted by addition of palladium reagents that trigger the cleavage of the spacer, and the release of unfunctional monomeric peptides.

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Keywords

Dimers DNA complex DNA-binding Palladium Transcription Binding peptide bZIP transcription factors Palladium mediated Self-immolative

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https://doi.org/10.1039/D1RA09180H

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© 2022 The Author(s). Published by the Royal Society of Chemistry. This Open Access Article is licensed under a Creative Commons Attribution-Non Commercial 3.0 Unported Licence (http://creativecommons.org/licenses/by-nc/3.0/).

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© 2022 The Author(s). Published by the Royal Society of Chemistry. This Open Access Article is licensed under a Creative Commons Attribution-Non Commercial 3.0 Unported Licence (http://creativecommons.org/licenses/by-nc/3.0/).

Except where otherwise noted, this item's license is described as © 2022 The Author(s). Published by the Royal Society of Chemistry. This Open Access Article is licensed under a Creative Commons Attribution-Non Commercial 3.0 Unported Licence (http://creativecommons.org/licenses/by-nc/3.0/).