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https://hdl.handle.net/2183/48713 Rapid detection of OXA-48-producing Enterobacteriaceae by matrix-assisted laser desorption ionization-time of flight mass spectrometry
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Authors
Oviaño, Marina
Barba, María José
Fernández, Begoña
Ortega, Adriana
Aracil, Belén
Oteo, Jesús
Campos, José
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Journal Title
Bibliographic citation
Oviaño M, Barba MJ, Fernández B, Ortega A, Aracil B, Oteo J, Campos J, Bou G. Rapid detection of OXA-48-producing Enterobacteriaceae by matrix-assisted laser desorption ionization-time of flight mass spectrometry. J Clin Microbiol. 2016 Mar;54(3):754-9.
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Abstract
[Abstract] A rapid and sensitive (100%) matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) assay was developed to detect OXA-48-type producers, using 161 previously characterized clinical isolates. Ertapenem was monitored to detect carbapenem resistance, and temocillin was included in the assay as a marker for OXA-48-producers. Structural analysis of temocillin is described. Data are obtained within 60 min.
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