Effects of Bisphenol a on the Microalga Chlamydomonas reinhardtii and the Clam Corbicula fluminea

Abstract

[Abstract] Bisphenol A (BPA) is used throughout the world and it could enter aquatic ecosystems causing harmful effects on humans, animals and plants. The current study relies on the investigation of the toxicity of this emerging pollutant on two freshwater species from different trophic levels: the microalga Chlamydomonas reinhardtii and the clam Corbicula fluminea. After 96 h of exposure to several concentrations of BPA, the growth of C. reinhardtii was affected, being the 96 h-EC50 value for growth 30 mg L−1. The toxicity and bioaccumulation of 30 mg L−1 BPA in microalgae after 24 h of exposure were studied. Several cytotoxicity biomarkers such as vitality, oxidative stress and cytoplasmic membrane potential were altered in exposed cells and microalgae accumulated 0.16 pg BPA cell−1. Regarding C. fluminea, four treatments were established: control without BPA (C); BPA in the food (microalgae pre-exposed for 24 h to 30 mg L−1) (M); BPA in the water (7.5 mg L−1) (W); BPA in both food and water (M + W). After one month of exposure, treated bivalves showed a significantly decrease in the filtration rate and increased lipid peroxidation levels, indicating fitness reduction and oxidative damage. Furthermore, the activities of catalase, glutathione reductase, Se-dependent and total glutathione peroxidase enzymes increased significantly in W and M + W treatments with respect to the control. Clams of the M + W treatment were the most affected, indicating that the little amount of BPA bioaccumulated by microalgae could increase the damage. Emerging contaminants may accumulate in several organisms, such as microalgae, and could have negative impacts on ecosystems.