Human cartilage engineering in an in vitro repair model using collagen scaffolds and mesenchymal stromal cells

Sanjurjo-Rodríguez, Clara; Castro Viñuelas, Rocío; Hermida Gómez, Tamara; Fuentes Boquete, Isaac Manuel; De-Toro, Javier; Blanco García, Francisco J; Díaz-Prado, Silvia

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http://hdl.handle.net/2183/19766

Human cartilage engineering in an in vitro repair model using collagen scaffolds and mesenchymal stromal cells

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Identifiers

URI: http://hdl.handle.net/2183/19766

Publication date

2017-09-28

Authors

Sanjurjo-Rodríguez, Clara

Castro Viñuelas, Rocío

Hermida Gómez, Tamara

Fuentes Boquete, Isaac Manuel

De-Toro, Javier

Blanco García, Francisco J

Díaz-Prado, Silvia

Bibliographic citation

Sanjurjo-Rodríguez C, Castro-Viñuelas R, Hermida-Gómez T, Fuentes-Boquete IM, de Toro FJ, Blanco FJ, Díaz-Prado SM. Human cartilage engineering in an in vitro repair model using collagen scaffolds and mesenchymal stromal cells. Int J Med Sci. 2017 Sep 28;14(12):1257-1262.

Abstract

[Abstract] The purpose of this study was to investigate cartilage repair of in vitro lesion models using human bone marrow mesenchymal stromal cells (hBMSCs) with different collagen (Col) scaffolds. Lesions were made in human cartilage biopsies. Injured samples were pre-treated with interleukin 1β (IL1β) for 24 h; also, samples were not pre-treated. hBMSCs were seeded on different types of collagen scaffolds. The resulting constructs were placed into the lesions, and the biopsies were cultured for 2 months in chondrogenic medium. Using the modified ICRSII scale, neotissues from the different scaffolds showed ICRS II overall assessment scores ranging from 50% (fibrocartilage) to 100% (hyaline cartilage), except for the Col I +Col II +HS constructs (fibrocartilage/hyaline cartilage, 73%). Data showed that hBMSCs cultured only on Col I +Col II +HS scaffolds displayed a chondrocyte-like morphology and cartilage-like matrix close to native cartilage. Furthermore, IL1β pre-treated biopsies decreased capacity for repair by hBMSCs and decreased levels of chondrogenic phenotype of human cartilage lesions.