Development of Conventional and Quantitative PCR Assays for the Detection of Vibrio europaeus Using a Species-Specific Gene Identified from the Pangenome

Rey-Varela, Diego; Diéguez, Ana L.; Rodríguez, Sergio; Dinçtürk, Ezgi; Villada, Antía; Ojea, Justa; Polo, David; Dubert, Javier

Use this link to cite:

https://hdl.handle.net/2183/48205

Development of Conventional and Quantitative PCR Assays for the Detection of Vibrio europaeus Using a Species-Specific Gene Identified from the Pangenome

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Polo_David_2026_Development_conventional_quantitative_PCR.pdf (1.16 MB)

Identifiers

URI: https://hdl.handle.net/2183/48205

DOI: 10.1016/j.jip.2026.108624

Publication date

2026-04-14

Authors

Rey-Varela, Diego

Diéguez, Ana L.

Rodríguez, Sergio

Dinçtürk, Ezgi

Villada, Antía

Ojea, Justa

Polo, David

Dubert, Javier

Bibliographic citation

Rey-Varela, D., Diéguez, A.L., Rodriguez, S., Dinçtürk, E., Villada, A., Ojea, J., Polo, D., Dubert, J., 2026. Development of conventional and quantitative PCR assays for the detection of Vibrio europaeus using a Species-Specific gene identified from the pangenome. Journal of Invertebrate Pathology 217, 108624. https://doi.org/10.1016/j.jip.2026.108624

Abstract

[Abstract] Vibriosis, specifically caused by Vibrio europaeus, significantly impacts bivalve aquaculture through severe mortalities mainly at early stages. Developing specific diagnostic tools has been hindered by a lack of reliable molecular targets. Using a pangenomic approach via our custom pipeline, PanGenAmp, we identified the gene ktrA as a highly specific marker. Novel conventional and quantitative PCR (qPCR) assays were developed based on ktrA, demonstrating high sensitivity and accuracy. These PCR tools fill a critical diagnostic gap, enabling early pathogen detection and improved biosecurity to mitigate the economic consequences of V. europaeus outbreaks in shellfish hatcheries.