Isolation and characterization of fourteen polymorphic microsatellite markers in the viperine snake Natrix maura

Le Chevalier, Hugo; Marí-Mena, Neus; Carro, Belén; Prunier, Jérôme G.; Bossu, Coralie; Darnet, Elodie; Souchet, Jérémie; Guillaume, Olivier; Calvez, Olivier; Bertrand, Romain; Barthe, Laurent; Pottier, Gilles; Martínez-Sylvestre, Albert; Verdaguer-Foz, Isabel; Mossoll-Torres, Marc; Trochet, Audrey; Aubret, Fabien

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http://hdl.handle.net/2183/24080

Isolation and characterization of fourteen polymorphic microsatellite markers in the viperine snake Natrix maura

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LeChevalier_Hugo_2019_Isolation_characterization_fourteen_polymorphic.pdf (393.71 KB)

Identifiers

URI: http://hdl.handle.net/2183/24080

Publication date

2019

Authors

Le Chevalier, Hugo

Marí-Mena, Neus

Carro, Belén

Prunier, Jérôme G.

Bossu, Coralie

Darnet, Elodie

Souchet, Jérémie

Guillaume, Olivier

Calvez, Olivier

Bertrand, Romain

Bibliographic citation

Le Chevalier H, Marí-Mena N, Carro B, et al. Isolation and characterization of fourteen polymorphic microsatellite markers in the viperine snake Natrix maura. Ecol Evol. 2019;00:1–5. https ://doi. org/10.1002/ece3.5623

Abstract

[Abstract] Nineteen polymorphic microsatellite loci were identified and developed for Natrix maura. Polymorphism was assessed for 120 individuals sampled across four sampling sites from the French Pyrenees Mountains. The number of alleles per locus ranged from 3 to 15, and expected heterozygosity per locus ranged from 0.227 to 0.863. We tested for deviation from Hardy–Weinberg equilibrium and linkage disequilibrium and assessed the presence of null alleles for all loci, resulting in a selection of 14 highquality polymorphic markers. These markers will be extremely useful in identifying fine-scale genetic structures and providing insight into conservation management plans of this species.