Generation of osteoarthritis and healthy mesenchymal cell lines for research on regenerative medicine for osteoarthritis

Abstract

[Purpose] Bone-marrow mesenchymal stem cells (BM-MSCs) are multipotent self-renewal adult cells with potential to regenerate the damaged tissues in degenerative diseases such as osteoarthritis (OA). Nevertheless, research require in vitro expansion of BM-MSCs, a process which eventually causes cell senescence. To overcome this problem cell lines can be used but, currently, BM-MSC lines available are scarce and present limitations regarding their differentiation capacities. For this reason, the aim of this study was to generate and characterize human BM-MSCs lines, derived from an OA patient and a healthy donor, with high chondrogenic and osteogenic capacities for their use in research on Regenerative Medicine for OA.